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Purified Mouse Anti-NCK
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken, Frog, Cow (Tested in Development)
Mouse IgG2b
Human NCK aa. 279-377
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
47 kDa
250 µg/ml
AB_397506
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610100 Rev. 2
Antibody Details
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108/NCK

NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors.  These proteins exert their effects by coupling activated receptors to downstream effectors.  NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity.  Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1.  Other proteins that directly interact with NCK are:  Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors.  The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells.  In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610100 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610100 Rev.2
Citations & References
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Development References (5)

  1. Dalva MB, Takasu MA, Lin MZ. EphB receptors interact with NMDA receptors and regulate excitatory synapse formation.. Cell. 2000; 103(6):945-956. (Clone-specific: Immunofluorescence). View Reference
  2. McManus MJ, Boerner JL, Danielsen AJ, Wang Z, Matsumura F, Maihle NJ. An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex.. J Biol Chem. 2000; 275(45):35328-35334. (Clone-specific: Western blot). View Reference
  3. Miyoshi-Akiyama T, Aleman LM, Smith JM, Adler CE, Mayer BJ. Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor.. Oncogene. 2001; 20(30):4058-4069. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  4. Roche S, McGlade J, Jones M, Gish GD, Pawson T, Courtneidge SA. Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent pathways.. EMBO J. 1996; 15(18):4940-4948. (Biology). View Reference
  5. Schmitz U, Thommes K, Beier I. Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck.. J Biol Chem. 2001 ; 276(25):22003-22010. (Clone-specific: Western blot). View Reference
View All (5) View Less
610100 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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