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Purified Mouse Anti-LIMK1
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Human LIMK1 aa. 232-333
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
72 kDa
250 µg/ml
AB_399224
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611749 Rev. 1
Antibody Details
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42/LIMK1

Two LIM motif-containing protein kinases (LIMK) have been identified, LIMK1 and LIMK2. These kinases contain two N-terminal LIM domains, a central PDZ domain, and a C-terminal Ser/Thr kinase domain. LIMK1 is highly expressed in brain, heart, and skeletal muscle, while LIMK2 exhibits the highest expression in placenta, liver, lung, kidney, and pancreas. LIMK1 is localized to the actin cytoskeleton and phosphorylates the actin binding/depolymerizing factor, cofilin. During Rho-induced neurite retraction, activation of ROCK leads to LIMK1 activation via phosphorylation at Thr508. In COS-7 cells, disruption of the  second LIM domain or the PDZ domain increases LIMK1-induced aggregation of the actin cytoskeleton. In addition, a 32 kDa splice variant that contains only the N-terminus (dLIMK1) suppresses LIMK1 activity by interaction with the C- terminal kinase domain. In humans, deletion of LIMK1 has been implicated in Williams syndrome, a disorder that produces a distinct cognitive profile and vascular disease. Thus, LIMK1, and its splice variant dLIMK1, are thought to have important roles in the regulation of the actin cytoskeleton in a wide varitey of tissues.

611749 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611749 Rev.1
Citations & References
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Development References (4)

  1. Edwards DC, Gill GN. Structural features of LIM kinase that control effects on the actin cytoskeleton. J Biol Chem. 1999; 274(16):11352-11361. (Biology). View Reference
  2. Frangiskakis JM, Ewart AK, Morris CA. LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition. Cell. 1996; 86(1):59-69. (Biology). View Reference
  3. Ohashi K, Nagata K, Maekawa M, Ishizaki T, Narumiya S, Mizuno K. Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop. J Biol Chem. 2000; 275(5):3577-3582. (Biology). View Reference
  4. Okano I, Hiraoka J, Otera H. Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs. J Biol Chem. 1995; 270.(52.):31321-31330. (Biology). View Reference
View All (4) View Less
611749 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.