Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
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Several gene regulatory proteins contain "zinc finger" domains of about 30 amino acids and N-terminal regions of about 75 amino acids, named the Krüppel-associated box A (KRAB-A). The KRAB-A domains have been implicated in transcriptional repression. KRIP-1 (KRAB-A-interacting protein 1) was identified as a result of its interaction with a KRAB-A domain. KRIP-1 is 834 amino acids with an apparent molecular weight of 105 kDa. Sequence alignments indicate that KRIP-1 shares identity with TIF-1, a transcriptional regulator of nuclear receptors. The region responsible for the transcriptional activity in KRIP-1 is located in the C-terminal region, which also contains a Cys4-His-Cys3 PHD finger domain.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (1)
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Kim SS, Chen YM, O'Leary E, Witzgall R, Vidal M, Bonventre JV. A novel member of the RING finger family, KRIP-1, associates with the KRAB-A transcriptional repressor domain of zinc finger proteins. Proc Natl Acad Sci U S A. 1996; 93(26):15299-15304. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.
