Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Cellular excitability is modulated by the precise function of voltage-sensitive ion channels. Large conductance potassium channels (Maxi-K) are unique in that they are sensitive to transmembrane potential and intracellular Ca2+ concentrations. These channels, important for neuronal firing and vascular tone, share many features with voltage-dependent Na+, Ca2+, and K+ channels. Among these are the S4 region, a motif with a repeated triple sequence of one positively charged amino acid and two hydrophobic amino acids. This region is thought to be the voltage sensor. Maxi-K is subject to complex metabolic control control that also involves G proteins and phosphorylation/dephosphorylation reactions. This type of K+ channel is composed of two subunits, the pore-forming α subunit (hslo) and the regulatory β subunit. The α subunit is subject to direct phosphorylation by cyclic GMP-dependent protein kinase (PKG) and dephosphorylation by protein phosphatase 2A. The fact that the α subunit of Maxi-K channels is a substrate of PKG supports the idea that these channels perform an important function in the cellular response to potent vasodilators, such as nitrocompounds and atrial natiuretic peptide.
Development References (4)
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Alioua A, Tanaka Y, Wallner M, et al. The large conductance, voltage-dependent, and calcium-sensitive K+ channel, Hslo, is a target of cGMP-dependent protein kinase phosphorylation in vivo. J Biol Chem. 1998; 273(49):32950-32956. (Biology). View Reference
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Diaz L, Meera P, Amigo J, et al. Role of the S4 segment in a voltage-dependent calcium-sensitive potassium (hSlo) channel. J Biol Chem. 1998; 273(49):32430-32436. (Biology). View Reference
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Meera P, Wallner M, Jiang Z, Toro L. A calcium switch for the functional coupling between alpha (hslo) and beta subunits (KV,Ca beta) of maxi K channels. FEBS Lett. 1996; 382(1-2):84-88. (Biology). View Reference
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Stefani E, Ottolia M, Noceti F, et al. Voltage-controlled gating in a large conductance Ca2+-sensitive K+channel (hslo). Proc Natl Acad Sci U S A. 1997; 94(10):5427-5431. (Biology). View Reference
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