Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152™).
Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152™).
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/resources/cellbiology/index.jsp
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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LSP-1 (Lymphocyte Specific Protein-1) is expressed in lymphocytes (B cells, cytotoxic T cells, and helper T cells), neutrophils, and macrophages and is involved in lymphocyte signal transduction. LSP-1 is a cytoplasmic membrane-bound protein that, following activation, translocates to the cytosol. This activation and translocation is mediated by phosphorylation of serine and threonine residues in the C-terminus of LSP-1 in response to concanavalin A or phorbal myristate acetate stimulation of murine T cells. LSP-1 contains two N-terminal calcium-binding sites and has also been shown to bind to actin. These results suggest that LSP-1 may be involved in the binding of actin to the plasma membrane following stimulation of PKC in murine T cells. The mRNA is constitutively expressed; however, the protein is only expressed following activation of the cell. Furthermore, LSP-1 is a substrate for MAPKAP kinase 2 (MAP kinase-activated protein kinase 2) in neutrophils.
Development References (5)
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Howard T, Li Y, Torres M, Guerrero A, Coates T. The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein. Blood. 1994; 83(1):231-241. (Biology). View Reference
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Jongstra-Bilen J, Young AJ, Chong R, Jongstra J. Human and mouse LSP1 genes code for highly conserved phosphoproteins. J Immunol. 1990; 144(3):1104-1110. (Biology). View Reference
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Kadiyala RK, McIntyre BW, Krensky AM. Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen. Eur J Immunol. 1990; 20(11):2417-2423. (Biology). View Reference
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Klein DP, Jongstra-Bilen J, Ogryzlo K, Chong R, Jongstra J. Lymphocyte-specific Ca2+-binding protein LSP1 is associated with the cytoplasmic face of the plasma membrane. Mol Cell Biol. 1989; 9(7):3043-3048. (Biology). View Reference
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Matsumoto N, Kojima S, Osawa T, Toyoshima S. Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes. J Biochem (Tokyo). 1994; 117(1):222-229. (Biology). View Reference
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