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Purified Mouse Anti-Human Exportin-1
Product Details
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BD Transduction Laboratories™
CRM1
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human hCRM1 aa. 2-122
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
112 kDa
250 µg/ml
AB_399313
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611833 Rev. 1
Antibody Details
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17/Exportin-1/CRM1

The nuclear pore complex (NPC) is a 125 MDa nuclear envelope complex that mediates nucleocytoplasmic shuttling of RNA and protein. NPC is comprised of more than 30 different proteins, including Ran/TC4, importins, and exportins . Exportin-1(hCRM1/XPO1) is the widely expressed human homologue of the yeast CRM1 protein, which functions in the maintenance of chromatin structure and nuclear export. Exportin-1 has an N-terminal CRIME domain that is homologous to yeast CRM1 and importin-β. Intracellular localization of Exportin-1 shows association with the NPC and overexpression of Exportin-1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus. Exportin-1 also binds other nuclear transport related proteins, such as RanGTP, snurportin-1, and the nucleoporins CAN/Nup214 and Nup50. Overexpression of CAN leads to sequestration of Exportin-1 in the nucleoplasm, which inhibits cell growth and induces apoptosis. In addition, Exportin-1 may export specific proteins, such as Rev and PKI, via binding to a leucine-rich nuclear export signal (NES). Thus, Exportin-1 is an important protein and RNA nuclear export receptor, which may be required for normal cell growth and survival.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611833 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611833 Rev.1
Citations & References
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Development References (5)

  1. Boer J, Bonten-Surtel J, Grosveld G. Overexpression of the nucleoporin CAN/NUP214 induces growth arrest, nucleocytoplasmic transport defects, and apoptosis. Mol Cell Biol. 1998; 18(3):1236-1247. (Biology). View Reference
  2. Fornerod M, Ohno M, Yoshida M, Mattaj IW. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell. 1997; 90(6):1051-1060. (Biology). View Reference
  3. Ishida N, Hara T, Kamura T, Yoshida M, Nakayama K, Nakayama KI. Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export. J Biol Chem. 2002; 277(17):14355-14358. (Clone-specific: Western blot). View Reference
  4. Massenet S, Pellizzoni L, Paushkin S, Mattaj IW, Dreyfuss G. The SMN complex is associated with snRNPs throughout their cytoplasmic assembly pathway. Mol Cell Biol. 2002; 22(18):6533-6541. (Clone-specific: Western blot). View Reference
  5. Paraskeva E, Izaurralde E, Bischoff FR, et al. CRM1-mediated recycling of snurportin 1 to the cytoplasm. J Cell Biol. 1999; 145(2):255-264. (Biology). View Reference
View All (5) View Less
611833 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.