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Purified Mouse Anti-Human DNMT1
Purified Mouse Anti-Human DNMT1

Western blot analysis of DNMT1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- human DNMT1 antibody.

Purified Mouse Anti-Human DNMT1

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Western blot analysis of DNMT1 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- human DNMT1 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).

Product Details
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BD Transduction Laboratories™
DNA Methyl Transferase-1
Human (QC Testing)
Mouse IgG2b
Human DNMT1 aa. 476-670
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
200 kDa
250 µg/ml
AB_399899
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
612619 Rev. 1
Antibody Details
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18/DNMT1

DNA methylation of the 5' position of the cytosine ring within CpG dinucleotides is essential for embryonic development. This methylation may affect transcriptional repression, formation of compact chromatin structures, X chromosome inactivation, and imprinting control. Three important DNA (Cytosine-5) methyltransferases (DNMTs) include DNMT1, DNMT3A, and DNMT3B. DNMT1 contains an N-terminal regulatory domain that may target it to replication foci, and a C-terminal catalytic domain that is homologous to bacterial cystosine 5-methylases. DNMT1 knockout mice have reduced DNA methylation, defects in imprinting, and derepression of endogenous retroviruses, and are embryonic lethal. DNMT1 is responsible for DNA methylations that occur after replication, while DNMT3A and DNMT3B act to establish new methylation patterns during embryogenesis. DNMT1 and DNMT3A are expressed in most fetal tissues, while DNMT3B is expressed only in fetal liver. All of these DNMTs are found in various adult tissues and show increased expression in some tumors and cancer cell lines. Thus, DNMT methylation of CpG dinucleotides is important for regulating DNA structure during development.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612619 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612619 Rev.1
Citations & References
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Development References (4)

  1. Robertson KD, Ait-Si-Ali S, Yokochi T. DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters. Nat Genet. 2000; 25(3):338-342. (Biology). View Reference
  2. Robertson KD, Uzvolgyi E, Liang G. The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors. Nucleic Acids Res. 1999; 27(11):2291. (Biology). View Reference
  3. Yen RW, Vertino PM, Nelkin BD. Isolation and characterization of the cDNA encoding human DNA methyltransferase. Nucleic Acids Res. 1992; 20(9):2287. (Biology). View Reference
  4. Yoder JA, Yen RW, Vertino PM, Bestor TH, Baylin SB. New 5' regions of the murine and human genes for DNA (cytosine-5)-methyltransferase. J Biol Chem. 1996; 271(49):31092-31097. (Biology). View Reference
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612619 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.