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Purified Mouse Anti- Human CD105
Purified Mouse Anti- Human CD105
Western blot analysis of CD105 on human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-CD105.
Purified Mouse Anti- Human CD105

Immunofluorescent staining of ES2 cells with anti-CD105/endoglin antibody.

Western blot analysis of CD105 on human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-CD105.

Immunofluorescent staining of ES2 cells with anti-CD105/endoglin antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1, κ
Human S-Endoglin aa. 24-144
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
95 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Antibody Details
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Endoglin (CD105), a major glycoprotein of human vascular endothelium, is a type I integral membrane protein with a large extracellular region, a hydrophobic transmembrane region, and a short cytoplasmic tail. It also contains an RGD tripeptide that may be important for cellular adhesion. There are two forms of endoglin (S-endoglin and L-endoglin) that differ in the length of their cytoplasmic tails. However, the isoforms may have similar functional activity. When overexpressed in fibroblasts, both form disulfide-linked homodimers via their extracellular domains. Endoglin binds TGF-β1 and TGF-β3 by associating with TGF-β type II receptor and binds BMP-7 by associating with activin type II receptor. Thus, endoglin is an accessory protein of multiple TGF-β superfamily kinase receptor complexes. Loss of function mutations in the human endoglin gene cause hereditary hemorrhagic telangiectasia, which is characterized by vascular malformations. Deletion of endoglin in mice leads to death due to defective vascular development. Thus, endoglin is an endothelial specific cell surface protein that may regulate angiogenesis through interactions with TGF-β superfamily kinase receptors.

This antibody is routinely tested by western blot analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (4)

  1. Barbara NP, Wrana JL, Letarte M. Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-beta superfamily. J Biol Chem. 1999; 274(2):584-594. (Biology). View Reference
  2. Bellon T, Corbi A, Lastres P. Identification and expression of two forms of the human transforming growth factor-beta-binding protein endoglin with distinct cytoplasmic regions. Eur J Biochem. 1993; 23(9):2340-2345. (Biology). View Reference
  3. Gougos A, Letarte M. Primary structure of endoglin, an RGD-containing glycoprotein of human endothelial cells. J Biol Chem. 1990; 265(15):8361-8364. (Biology). View Reference
  4. Li DY, Sorensen LK, Brooke BS. Defective angiogenesis in mice lacking endoglin. Science. 1999; 284(5419):1534-1537. (Biology). View Reference
View All (4) View Less
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.