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Purified Mouse Anti-GS28
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat (Tested in Development)
Mouse IgG2a
Rat GS28 aa. 3-108
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
28 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611185 Rev. 1
Antibody Details
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Most vesicular fusion events require the function of N-ethylmaleimide-sensitive factor (NSF) and the soluble NSF attachment proteins (SNAPs). Their function is dependent upon the SNAP receptor (SNARE) family of proteins. The specificity of vesicle docking and fusion is mediated by specific interactions between v-SNAREs on vesicles and t-SNAREs on target membranes. GS28 (Golgi SNARE with a size of 28 kDa) is a SNARE that associates with the cis-Golgi and participates in trafficking between the ER and the Golgi and between Golgi compartments. The majority of GS28, the first 230 and 250 aa, is thought to be anchored to the membrane via the C-terminal hydrophobic tail, which is formed by the remaining 20 aa. GS28 and syntaxin 5, another SNARE, exist as a protein complex in the Golgi and this complex is important for the function of both proteins in ER-Golgi transport. The GS28/syntaxin 5 complex can be dissociated by α-SNAP and NSF. In addition, GS28 is thought to interact with α-SNAP when the GS28/syntaxin 5 complex is dissociated. Thus, GS28 is a SNARE protein that mediates, in complex with syntaxin 5, transport within the Golgi and between Golgi and ER.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611185 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611185 Rev.1
Citations & References
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Development References (5)

  1. Hamza I, Prohaska J, Gitlin JD. Essential role for Atox1 in the copper-mediated intracellular trafficking of the Menkes ATPase. Proc Natl Acad Sci U S A. 2003; 100(3):1215-1220. (Biology: Fluorescence microscopy, Immunofluorescence). View Reference
  2. Lanoix J, Ouwendijk J, Stark A, et al. Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1. J Cell Biol. 2001; 155(7):1199-1212. (Biology: Western blot). View Reference
  3. Muller JM, Shorter J, Newman R, et al. Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion. J Cell Biol. 2002; 157(7):1161-1173. (Biology: Immunoprecipitation, Western blot). View Reference
  4. Subramaniam VN, Loh E, Hong W. N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5 Golgi SNAP receptors (SNARE) complex. J Biol Chem. 1997; 272(41):25441-25444. (Biology). View Reference
  5. Subramaniam VN, Peter F, Philp R, Wong SH, Hong W. GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport. Science. 1996; 272(5265):1161-1163. (Biology). View Reference
View All (5) View Less
611185 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.