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Purified Mouse Anti-GS27
Purified Mouse Anti-GS27

Western blot analysis of GS27 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-GS27 antibody.

Purified Mouse Anti-GS27

Immunofluorescence staining of human endothelial cells.

Western blot analysis of GS27 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-GS27 antibody.

Immunofluorescence staining of human endothelial cells.

Product Details
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BD Transduction Laboratories™
Golgi SNARE of 27 kDa; Membrin
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human GS27 aa. 5-124
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
27 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611034 Rev. 1
Antibody Details
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25/GS27

Eukaryotic protein trafficking involves the packaging of target molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Components of both the vesicle and the synaptic plasma membrane interact to form a fusion complex that mediates vesicle docking and fusion. This fusion complex contains NSF (N-ethyl-maleimide-sensitive factor), SNAPs (soluble NSF attachment proteins), and receptor proteins (SNAREs) that include synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosome-associated protein of 25 kDa). Interactions between vesicle SNAREs and target membrane SNARES mediates the specificity of docking. Along one pathway of protein trafficking, the Golgi apparatus receives proteins from the ER. These proteins move from cis-Golgi to trans-Golgi, through a stack of cisternae, towards the trans-Golgi network. From here, they are delivered to their proper destination in the cell. GS27 (Golgi SNARE of 27 kDa) (membrin) is a Golgi-associated SNARE. It functions in medial-to-trans-Golgi protein movement.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (3)

  1. Hay JC, Chao DS, Kuo CS, Scheller RH. Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells. Cell. 1997; 89(1):149-158. (Biology). View Reference
  2. Hay JC, Klumperman J, Oorschot V, Steegmaier M, Kuo CS, Scheller RH. Localization, dynamics, and protein interactions reveal distinct roles for ER and Golgi SNAREs. J Cell Biol. 1998; 141(7):1489-1502. (Biology). View Reference
  3. Lowe SL, Peter F, Subramaniam VN, Wong SH, Hong W. A SNARE involved in protein transport through the Golgi apparatus. Nature. 1997; 389(6653):881-884. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.