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Purified Mouse Anti-Gephyrin
Purified Mouse Anti-Gephyrin

Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Gephyrin.

Purified Mouse Anti-Gephyrin

Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, 20X.

Western blot analysis of Gephyrin on rat brain lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-Gephyrin.

Rat cerebellum, formalin-fixed paraffin embedded tissue, with citrate pre-treatment, 20X.

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Chicken (Tested in Development)
Mouse IgG1
Rat Gephyrin aa. 569-726
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
93 kDa
250 µg/ml
AB_397929
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610584 Rev. 1
Antibody Details
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45/Gephyrin

The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610584 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610584 Rev.1
Citations & References
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Development References (4)

  1. Feng G, Tintrup H, Kirsch J, et al. Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science. 1998; 282(5392):1321-1324. (Clone-specific: Western blot). View Reference
  2. Kneussel M, Haverkamp S, Fuhrmann JC, et al. The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse. Proc Natl Acad Sci U S A. 2000; 97(15):8594-8599. (Clone-specific: Immunofluorescence, Western blot). View Reference
  3. Meyer G, Kirsch J, Betz H, Langosch D. Identification of a gephyrin binding motif on the glycine receptor beta subunit. Neuron. 1995; 15(3):563-572. (Biology). View Reference
  4. Prior P, Schmitt B, Grenningloh G, et al. Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein. Neuron. 1992; 8(6):1161-1170. (Biology). View Reference
View All (4) View Less
610584 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.