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Purified Mouse Anti-FKBP65
Purified Mouse Anti-FKBP65

Western blot analysis of FKBP65 on a PC-12 cell lysate (Rat neuroblastoma; ATCC CRL-1721) using

0.5 µg/mL of the Mouse Anti-FKBP65 antibody.

Purified Mouse Anti-FKBP65

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of FKBP65 on a PC-12 cell lysate (Rat neuroblastoma; ATCC CRL-1721) using

0.5 µg/mL of the Mouse Anti-FKBP65 antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
FK506 Binding Protein-65
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Mouse FKBP65 aa. 434-576
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
68-72 kDa
250 µg/ml
AB_397975
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610648 Rev. 2
Antibody Details
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25/FKBP65

FK506, one of multiple potent compounds that block T cell proliferation, is used for immunosuppressive therapy.  Several FK506-binding proteins (FKBPs) have been identified in the search for intracellular targets for FK506.  These include FKBP12, FKBP13, FKBP51, and FKBP52.  The immunosuppressive effects of FK506 and FKBPs result from the inhibition of the calcineurin phosphatase, a well known component of the signaling cascade leading to IL-2 production.  FKBP65 is another member of the FKBP protein family and contains the characteristic peptidylprolyl cis-trans-isomerase activity.  FKBP65 has been reported to be expressed in the lung, testis, brain, heart, and spleen.  Although it has a predicted molecular weight of 65 kDa, FKBP65 has been observed to migrate ranging from 68-72 kDa in SDS electrophoresis gels, perhaps, due to glycosylation and/or phosphorylation.

610648 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610648 Rev.2
Citations & References
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Development References (2)

  1. Coss MC, Winterstein D, Sowder RC 2nd, Simek SL. Molecular cloning, DNA sequence analysis, and biochemical characterization of a novel 65-kDa FK506-binding protein (FKBP65). J Biol Chem. 1995; 270(49):29336-29341. (Biology). View Reference
  2. Patterson CE, Schaub T, Coleman EJ, Davis EC. Developmental regulation of FKBP65. An ER-localized extracellular matrix binding-protein. Mol Biol Cell. 2000; 11(11):3925-3935. (Biology: Immunofluorescence, Immunohistochemistry, Western blot). View Reference
610648 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.