Skip to main content Skip to navigation
Purified Mouse Anti-FBP
Purified Mouse Anti-FBP

Western blot analysis of FBP on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-FBP antibody.

Purified Mouse Anti-FBP

Immunofluorescence staining of HCT-8 cells (Human colorectal adenocarcinoma; ATCC CCL-244).

Western blot analysis of FBP on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-FBP antibody.

Immunofluorescence staining of HCT-8 cells (Human colorectal adenocarcinoma; ATCC CCL-244).

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
FUSE Binding Protein
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human FBP aa. 61-180
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
74 kDa
250 µg/ml
AB_398814
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611286 Rev. 1
Antibody Details
Down Arrow Up Arrow
6/FBP

The c-myc proto-oncogene is an essential participant in regulation of normal cell proliferation and programmed cell death. The far-upstream FUSE Binding Protein (FBP) is one of many factors that bind to a single strand of DNA in the 5' region of the c-myc gene. FBP, FBP2, and FBP3 comprise a family of single-strand DNA binding proteins that possess many features found in more conventional transcription factors. All FBP proteins bind specifically to a single strand of FUSE via a centrally located DNA binding domain and all contain a potent C-terminal transactivation domain. The transactivation domain of FBP contains three motif copies composed of tyrosine diads. However, a single tyrosine motif is sufficient to activate transcription. The N-terminal portion of FBP can repress the C-terminal transactivation potential. This repression is thought to be mediated by interactions between N- and C-terminal domains that produce an inactive conformation. Although FBP2 and FBP3 exhibit no specific pattern of expression, FBP expression mimics that of c-myc. These data implicate FBP as a transcription factor that is important for the regulation of cell growth and differentiation.

611286 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611286 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Davis-Smyth T, Duncan RC, Zheng T, Michelotti G, Levens D. The far upstream element-binding proteins comprise an ancient family of single-strand DNA-binding transactivators. J Biol Chem. 1996; 271(49):31679-31687. (Biology). View Reference
  2. Duncan R, Bazar L, Michelotti G. A sequence-specific, single-strand binding protein activates the far upstream element of c-myc and defines a new DNA-binding motif. Genes Dev. 1994; 8(4):465-480. (Biology). View Reference
  3. Duncan R, Collins I, Tomonaga T, Zhang T, Levens D. A unique transactivation sequence motif is found in the carboxyl-terminal domain of the single-strand-binding protein FBP. Mol Cell Biol. 1996; 16(5):2274-2282. (Biology). View Reference
611286 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.