Skip to main content Skip to navigation
Purified Mouse Anti-ERK2
Purified Mouse Anti-ERK2

Western blot analysis of ERK2 on rat pituitary lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of ERK2.

Purified Mouse Anti-ERK2

ERK 2 (clone 33) staining on rat brain. Formalin fixed paraffin section with citrate

buffer pretreatment. 20X

Purified Mouse Anti-ERK2

Immunofluorescence staining of MCF7 cells.

Western blot analysis of ERK2 on rat pituitary lysate.  Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of ERK2.

ERK 2 (clone 33) staining on rat brain. Formalin fixed paraffin section with citrate

buffer pretreatment. 20X

Immunofluorescence staining of MCF7 cells.

Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken, Frog (Tested in Development)
Mouse IgG2b
Rat ERK2 aa. 219-358
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
42 kDa
250 µg/ml
AB_397510
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Antibody Details
Down Arrow Up Arrow
33/ERK2

The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) are activated after cell stimulation by a wide variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. Structural analysis of ERK2 indicates that phosphorylation induces a conformational change that exposes the active site for substrate binding. Myriad proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK2 is the 42kDa member of the ERK family and is highly homologous to ERK1.

Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Kim SJ, Ju JW, Oh CD. ERK-1/2 and p38 kinase oppositely regulate nitric oxide-induced apoptosis of chondrocytes in association with p53, caspase-3, and differentiation status. J Biol Chem. 2002; 277(2):1332-1339. (Clone-specific: Western blot). View Reference
  2. Lehmann K, Janda E, Pierreux CE, et al. Raf induces TGFbeta production while blocking its apoptotic but not invasive responses: a mechanism leading to increased malignancy in epithelial cells. Genes Dev. 2000; 14(20):2610-2622. (Clone-specific: Western blot). View Reference
  3. Liu L, Tsai JC, Aird WC. Egr-1 gene is induced by the systemic administration of the vascular endothelial growth factor and the epidermal growth factor. Blood. 1772; 96(5):1772-1781. (Clone-specific: Western blot). View Reference
  4. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Clone-specific: Flow cytometry, Immunoprecipitation, Western blot). View Reference
  5. Visconti R, Gadina M, Chiariello M. Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity. Blood. 2000; 96(5):1844-1852. (Clone-specific: Immunoprecipitation, In vitro kinase assay, Western blot). View Reference
View All (5) View Less

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.