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Purified Mouse Anti-Dynamin
Purified Mouse Anti-Dynamin

Western blot analysis of Dynamin on PC12 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Dynamin.

Purified Mouse Anti-Dynamin

Dynamin (clone 41) staining on rat brain. Formalin fixed paraffin section without citrate buffer pretreatment. 40X.

Western blot analysis of Dynamin on PC12 cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Dynamin.

Dynamin (clone 41) staining on rat brain. Formalin fixed paraffin section without citrate buffer pretreatment. 40X.

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat Dynamin I aa. 698-851
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
100 kDa
250 µg/ml
AB_397640
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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41/Dynamin

Dynamin is a nerve terminal phosphoprotein with intrinsic GTPase activity which plays an important role in endocytosis. This GTPase activity is stimulated in vitro by microtubules, SH3 domain-containing proteins, phospholipids, and Protein Kinase C (PKC)-mediated phosphorylation. There are at least two distinct dynamin genes in mammals, which show 79% identity, encoding proteins Dynamin I and Dynamin II. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. Depolarization stimulates synaptic vesicle recycling and Dynamin I is subsequently dephosphorylated. This rapid dephosphorylation is mediated by calcineurin which acts as a switch for depolarization-initiated synaptic vesicle endocytosis. The C-terminus of dynamin contains two clusters of proline-rich SH3 domain binding proline motifs which interact with known SH3 domain proteins during tyrosine kinase receptor activation. It is reported that clone 41 detects both dynamin I and dynamin II.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (5)

  1. Hayes S, Chawla A, Corvera S. TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2. J Cell Biol. 2002; 158(7):1239-1249. (Clone-specific: Immunofluorescence). View Reference
  2. Kalthoff C, Groos S, Kohl R, Mahrhold S, Ungewickell EJ. Clint: a novel clathrin-binding ENTH-domain protein at the Golgi. Mol Biol Cell. 2002; 13(11):4060-4073. (Clone-specific). View Reference
  3. Ochoa GC, Slepnev VI, Neff L, et al. A functional link between dynamin and the actin cytoskeleton at podosomes. J Cell Biol. 2000; 150(2):377-389. (Clone-specific: Western blot). View Reference
  4. Scaife R, Gout I, Waterfield MD, Margolis RL. Growth factor-induced binding of dynamin to signal transduction proteins involves sorting to distinct and separate proline-rich dynamin sequences. EMBO J. 1994; 13(11):2574-2582. (Biology). View Reference
  5. Schmidlin F, Dery O, DeFea KO, et al. Dynamin and Rab5a-dependent trafficking and signaling of the neurokinin 1 receptor. J Biol Chem. 2001; 276(27):25427-25437. (Clone-specific: Immunofluorescence, Western blot). View Reference
View All (5) View Less
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.