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Purified Mouse Anti-Coilin
Purified Mouse Anti-Coilin

Western blot analysis of Coilin on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243).  Lane 1: 1:500, lane 1: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Coilin antibody.

Purified Mouse Anti-Coilin

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2).

 

Western blot analysis of Coilin on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243).  Lane 1: 1:500, lane 1: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Coilin antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2).

 

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human Coilin aa. 226-332
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
80 kDa
250 µg/ml
AB_2081554
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612074 Rev. 1
Antibody Details
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56/Coilin

Ramon Cajal described small silver-staining organelles in the nuclei of pyramidal neurons, which have been referred to as cajal bodies or coiled bodies. An 80 kDa protein called p80 coilin was identified through its co-localization to coiled bodies. Coilin is a molecular marker of coiled bodies, but can also be found in the nucleoplasm, and may shuttle back and forth between the cytoplasm and nucleoplasm. Studies of GFP-coilin show that coiled bodies can move within the nucleoplasm to and from nucleoli. Other proteins that have been co-localized with coilin to the coiled bodies include cell cycle proteins, snRNPs, U3 snRNA, U7 snRNA, and several nucleolar proteins. Coiled bodies may recruit U7 snRNP and  the stem-loop-binding protein to the chromosomal sites of histone gene transcription. Possibly, coilin is important for the formation of coiled bodies that act as sites for preassembly of "transcriptosomes", which facilitate gene transcription and RNA processing.

612074 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612074 Rev.1
Citations & References
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Development References (2)

  1. Andrade LE, Chan EK, Raska I, Peebles CL, Roos G, Tan EM. Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin. J Exp Med. 1991; 173(6):1407-1419. (Biology). View Reference
  2. Bellini M, Gall JG. Coilin shuttles between the nucleus and cytoplasm in Xenopus oocytes. Mol Biol Cell. 1999; 10(10):3425-3434. (Biology). View Reference
612074 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.