Immunofluorescence staining on human fibroblasts.
Immunofluorescence staining on human fibroblasts.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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The human homologue of the Saccharomyces cerevisiae CDC34 gene appears to be a highly conserved, single copy gene that is expressed in many cell lines. The human CDC34 cDNA can functionally substitute for the yeast CDC34 gene and, therefore, is a mammalian homologue of a group of yeast genes (CDC34, CDC4, and CDC53) required for the late G1-S phase transition. In yeast, CDC34 is a member of the large family of ubiquitin-conjugating ligases (UBC) that are involved in linking ubiquitin polypeptides to target proteins. It is thought that the role of CDC34 in cell cycle progression is related to the ubiquitination of G1 cyclins. In addition, p53 and other proteins involved in growth control such as Fos, Myc, and Mos are targeted for proteolysis by ubiquitin-dependent mechanisms. Absence of CDC34 causes yeast cell cycle arrest prior to DNA synthesis. Thus, it will be important to determine the role of CDC34 and the other proteins involved in the G1-S transition in mammalian cells. This will be a significant step toward understanding the regulation of the initiation of DNA synthesis, as well as the mechanism controlling the cell cycle transition following DNA damage.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Eliseeva E, Pati D, Diccinanni MB, Yu AL. Expression and localization of the CDC34 ubiquitin-conjugating enzyme in pediatric acute lymphoblastic leukemia. Cell Growth Differ. 2001; 12(8):427-433. (Biology: Immunohistochemistry, Western blot). View Reference
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Hagglund R, Van Sant C, Lopez P, Roizman B. Herpes simplex virus 1-infected cell protein 0 contains two E3 ubiquitin ligase sites specific for different E2 ubiquitin-conjugating enzymes. Proc Natl Acad Sci U S A. 2002; 99(2):631-636. (Biology: Western blot). View Reference
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Plon SE, Leppig KA, Do HN, Groudine M. Cloning of the human homolog of the CDC34 cell cycle gene by complementation in yeast. Proc Natl Acad Sci U S A. 1993; 90(22):10484-10488. (Biology). View Reference
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Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology: Immunofluorescence). View Reference
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Topper LM, Bastians H, Ruderman JV, Gorbsky GJ.. Elevating the level of Cdc34/Ubc3 ubiquitin-conjugating enzyme in mitosis inhibits association of CENP-E with kinetochores and blocks the metaphase alignment of chromosomes. J Cell Biol. 2001; 154(4):707-714. (Biology: Western blot). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
