The human homologue of the Saccharomyces cerevisiae CDC34 gene appears to be a highly conserved, single copy gene that is expressed in many cell lines. The human CDC34 cDNA can functionally substitute for the yeast CDC34 gene and, therefore, is a mammalian homologue of a group of yeast genes (CDC34, CDC4, and CDC53) required for the late G1-S phase transition. In yeast, CDC34 is a member of the large family of ubiquitin-conjugating ligases (UBC) that are involved in linking ubiquitin polypeptides to target proteins. It is thought that the role of CDC34 in cell cycle progression is related to the ubiquitination of G1 cyclins. In addition, p53 and other proteins involved in growth control such as Fos, Myc, and Mos are targeted for proteolysis by ubiquitin-dependent mechanisms. Absence of CDC34 causes yeast cell cycle arrest prior to DNA synthesis. Thus, it will be important to determine the role of CDC34 and the other proteins involved in the G1-S transition in mammalian cells. This will be a significant step toward understanding the regulation of the initiation of DNA synthesis, as well as the mechanism controlling the cell cycle transition following DNA damage.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.