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Purified Mouse Anti-ATP Synthase α
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Human ATP Synthase α aa. 113-220
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
55 kDa
250 µg/ml
AB_399818
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612516 Rev. 1
Antibody Details
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51/ATP Synthase α

ATP synthase is a large enzyme complex that uses an electrochemical H+ or Na+ gradient to synthesize ATP from ADP and Pi, providing the organism with the  ATP needed for energy. The complex consists of two major units, F0 and F1. F0 is embedded in the inner membrane of the mitochondria and, due to its hydrophobic nature, translocates protons across this membrane. F1 is the catalytic portion in the matrix region of the mitochondria and is comprised of α, β, γ, δ, and ε subunits at a 3:3:1:1:1 ratio. The α subunit is a ubiquitous protein that is highly conserved among species. It has an adenine specific binding site that binds both ATP and ADP. There are two glycine rich regions in the sequence, the A domain and B domain, that are thought to be part of the nucleotide binding domain. It has been demonstrated that the N-terminus of the α subunit is necessary for the correct functional and structural connection of F0 to F1. Thus, the α subunit is an essential component of the ATP synthase complex and plays a role in properly orienting the F0 and F1 units.

612516 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612516 Rev.1
Citations & References
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Development References (3)

  1. Lee JH, Garboczi DN, Thomas PJ, Pedersen PL. Mitochondrial ATP synthase. cDNA cloning, amino acid sequence, overexpression, and properties of the rat liver alpha subunit. J Biol Chem. 1990; 265(8):4664-4669. (Biology). View Reference
  2. Xu T, Zanotti F, Gaballo A, Raho G, Papa S. F1 and F0 connections in the bovine mitochondrial ATP synthase: the role of the of alpha subunit N-terminus, oligomycin-sensitivity conferring protein (OCSP) and subunit d. Eur J Biochem. 2000; 267(14):4445-4455. (Biology). View Reference
  3. von Ballmoos C, Appoldt Y, Brunner J, Granier T, Vasella A, Dimroth P. Membrane topography of the coupling ion binding site in Na+-translocating F1F0 ATP synthase. J Biol Chem. 2001; 277(5):3504-3510. (Biology). View Reference
612516 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.