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Purified Mouse Anti-Amphiphysin
Purified Mouse Anti-Amphiphysin

Western blot of Amphiphysin on a rat cerebrum lysate. Lane 1: 1:10,000, lane 2: 1: 20,000, lane 3: 1:40,000 dilution of the mouse anti-Amphiphysin antibody.

Purified Mouse Anti-Amphiphysin

Immunohistochemical staining for Amphiphysin on a formalin-fixed paraffin-embedded section, with citrate buffer pretreatment, on rat brain (40X magnification).

Western blot of Amphiphysin on a rat cerebrum lysate. Lane 1: 1:10,000, lane 2: 1: 20,000, lane 3: 1:40,000 dilution of the mouse anti-Amphiphysin antibody.

Immunohistochemical staining for Amphiphysin on a formalin-fixed paraffin-embedded section, with citrate buffer pretreatment, on rat brain (40X magnification).

Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Human Amphiphysin aa. 258-414
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
125 kDa
250 µg/ml
AB_398037
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610714 Rev. 1
Antibody Details
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15/Amphiphysin

Amphiphysin was originally described in chicken brain and located at the presynaptic terminal. It was also identified as the major autoantigen in Stiff-Man Syndrome (SMS), a neurological disease linked to an autoimmune response to antigens in the central nervous system. Amphiphysin interacts with several proteins important in the synaptic vesicle cycle. Amphiphysin contains a Src homology 3 (SH3) domain that binds to the C-terminal and proline-rich region of dynamin at the synapse. Microinjection of amphiphysin inhibits synaptic vesicle endocytosis. In addition, amphiphysin binds to AP-2 at the plasma membrane and to the inositol 5-phosphatase, synaptojanin. The interaction between synaptojanin and amphiphysin also appears to be mediated through amphiphysin's SH3-domain.

610714 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610714 Rev.1
Citations & References
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Development References (4)

  1. David C, Solimena M, De Camilli P. Autoimmunity in stiff-Man syndrome with breast cancer is targeted to the C-terminal region of human amphiphysin, a protein similar to the yeast proteins, Rvs167 and Rvs161. FEBS Lett. 1994; 351(1):73-79. (Biology). View Reference
  2. Drake MT, Traub LM. Interaction of two structurally distinct sequence types with the clathrin terminal domain beta-propeller. J Biol Chem. 2001; 276(31):28700-28709. (Biology: Western blot). View Reference
  3. Traub LM, Downs MA, Westrich JL, Fremont DH. Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly. Proc Natl Acad Sci U S A. 1999; 96(16):8907-8912. (Biology: Western blot). View Reference
  4. Wang LH, Sudhof TC, Anderson RG. The appendage domain of alpha-adaptin is a high affinity binding site for dynamin. J Biol Chem. 1995; 270(17):10079-10083. (Biology). View Reference
View All (4) View Less
610714 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.