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Purified Mouse Anti-Akt
Purified Mouse Anti-Akt

Western blot analysis of Akt on a HCT-8 (human colorectal adenocarcinoma) cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Akt antibody.

Purified Mouse Anti-Akt

Immunofluorescence staining on WI-38 cells (human lung fibroblasts; ATCC CCL-75).

Western blot analysis of Akt on a HCT-8 (human colorectal adenocarcinoma) cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- Akt antibody.

Immunofluorescence staining on WI-38 cells (human lung fibroblasts; ATCC CCL-75).

Product Details
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BD Transduction Laboratories™
PKBα; RAC-PK
Human (QC Testing), Mouse, Rat, Dog, Fly (Tested in Development)
Mouse IgG2a
Human PKBα/Akt1 aa. 1-480
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
59 kDa
250 µg/ml
AB_398193
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610877 Rev. 1
Antibody Details
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2/PKBa/Akt

Protein Kinase B (PKB), also known as Rac (Related to the A and C kinases) Protein Kinase,  shows 73% and 68% to Protein Kinase C (PKC) and Protein Kinase A (PKA), respectively.  It was also identified as the product of the v-akt oncogene from T-cell lymphomas. Akt/PKB mRNA has been reported to be expressed in a wide variety of cell lines and has been detected in porcine brain, heart, liver, kidney, muscle, and ovary. Rac phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt/PKB is activated in response to growth factors through the activation of PI3-kinase and Ras. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate which may induce the membrane translocation of Akt/PKB coincident with its phosphorylation and activation. Cellular stress leading to the activation of the p38 MAP kinase also induces Akt/PKB activation, indicating a multiplicity of signaling pathways that activate Akt/PKB. Upon activation, Akt/PKB associates with some members of the PKC family of kinases, such as PKCδ and PKCζ. Downstream of Akt/PKB, GSK3 is thus far the only identified substrate for Akt/PKB phosphorylated in vivo.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610877 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610877 Rev.1
Citations & References
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Development References (2)

  1. Jones PF, Jakubowicz T, Pitossi FJ, Maurer F, Hemmings BA. Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Proc Natl Acad Sci U S A. 1991; 88(10):4171-4175. (Biology). View Reference
  2. Marte BM, Downward J. PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond. Trends Biochem Sci. 1997; 22(9):355-358. (Biology). View Reference
610877 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.