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FITC Mouse Anti-p120 Catenin
Product Details
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BD Transduction Laboratories™
pp120 (Src Substrate); p120cas
Human (QC Testing), Mouse, Rat, Chicken, Dog (Tested in Development)
Mouse IgG1
Mouse pp120 aa. 326-632
Immunofluorescence (Routinely Tested)
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to for technical protocols.
610135 Rev. 1
Antibody Details
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The membrane associated protein p120 Catenin (pp120 (Src substrate); p120cas) was identified as a tyrosine kinase substrate that is phosphorylated in Src transformed cells or in response to growth factor stimulation.  It shares structural similarity with the Drosophila Armadillo protein and the vertebrate β-catenin and γ-catenin proteins.  This similarity is evidenced by its characteristic Arm domain that is composed of 42-amino acid motif repeats.  In the cell, p120 Catenin is localized to the E-Cadherin/catenins cell adhesion complex.  Like β- and γ-catenin, p120 Catenin directly associates with the cytoplasmic C-terminus of E-Cadherin via its Arm domain and may similarly interact with other Cadherins.  It exists as four isoforms that range in size from 90-115 kDa.  Expression of these isoforms is heterogeneous in human carcinomas, suggesting that altered pp120 expression contributes to malignancy due to loss of functional cell adhesions.  Multiple tyrosine residues (Y96, Y112, Y228, Y280, Y257, Y291, Y296, and Y302) in p120 Catenin are phosphorylated by Src and these phosphorylations may facilitate interaction with PTP1C/SHP-1 in response to EGF stimulation.  Thus, p120 Catenin is an Arm domain protein that interacts with both cell adhesion molecules, such as cadherins and cell signaling molecules, such as PTP1C.

610135 Rev. 1
Format Details
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Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Blue 488 nm
494 nm
518 nm
610135 Rev.1
Citations & References
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Development References (5)

  1. Daniel JM, Reynolds AB. The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha-catenin. Mol Cell Biol. 1995; 15(9):4819-4824. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Mo YY, Reynolds AB. Identification of murine p120 isoforms and heterogeneous expression of p120cas isoforms in human tumor cell lines. Cancer Res. 1996; 56(11):2633-2640. (Biology: Immunoprecipitation, Western blot). View Reference
  3. Reynolds AB, Daniel J, McCrea PD, Wheelock MJ, Wu J, Zhang Z. Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes. Mol Cell Biol. 1994; 14(12):8333-8342. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Shimazui T, Schalken JA, Giroldi LA, et al. Prognostic value of cadherin-associated molecules (alpha-, beta-, and gamma-catenins and p120cas) in bladder tumors. Cancer Res. 1996; 56(18):4154-4158. (Biology: Immunohistochemistry). View Reference
  5. Xu X, Li WE, Huang GY, et al. Modulation of mouse neural crest cell motility by N-cadherin and connexin 43 gap junctions. J Cell Biol. 2001; 154(1):217-229. (Clone-specific: Immunofluorescence). View Reference
View All (5) View Less
610135 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.