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FITC Mouse Anti- E-Cadherin
FITC Mouse Anti- E-Cadherin

LEFT IMAGE: Immunofluorescence staining of A431 cells.

RIGHT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182). E-Cadherin is observable at 120kDa. Left Panel: A431 lysate (ATCC CRL-1555; Human epithelial carcinoma) was blotted at 1:10000 & 1:20000 (Lanes 1 & 2 respectively; 30 second exposure). Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 (Lanes 1 & 2 respectively; 30 second exposure). Middle Right Panel: 293F cells transfected with human E-Cadherin (CDH1) was blotted at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure). Right Panel: 293 cells transfected with human P-Cadherin (CDH3) was blotted using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182) at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure).

LEFT IMAGE: Immunofluorescence staining of A431 cells.

RIGHT IMAGE: Western blot analysis of E-Cadherin using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182). E-Cadherin is observable at 120kDa. Left Panel: A431 lysate (ATCC CRL-1555; Human epithelial carcinoma) was blotted at 1:10000 & 1:20000 (Lanes 1 & 2 respectively; 30 second exposure). Middle Left Panel: 293F control lysate was blotted at 1:250 & 1:500 (Lanes 1 & 2 respectively; 30 second exposure). Middle Right Panel: 293F cells transfected with human E-Cadherin (CDH1) was blotted at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure). Right Panel: 293 cells transfected with human P-Cadherin (CDH3) was blotted using Purified Mouse Anti-E-Cadherin (Cat. No. 610181/610182) at 1:2500 & 1: 5000 (Lanes 1 & 2 respectively; 5 second exposure).

Product Details
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BD Transduction Laboratories™
CD324; CDH1; CADH1; Cadherin-1; ECAD; CDHE; Arc-1; LCAM; UVO; Uvomorulin
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG2a, κ
Human E-Cadherin C-terminal Recombinant Protein
Immunofluorescence (Routinely Tested), Immunohistochemistry, Immunoprecipitation (Not Recommended)
120 kDa
250 µg/ml
AB_2076677
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
612131 Rev. 2
Antibody Details
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36/E-Cadherin

E-Cadherin is a 120-kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells.  There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins.  In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation.  Its association with catenins is necessary for cell-cell adhesion.  These E-cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques.  Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties.  E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas.  Increased expression of E-Cadherin in these cells reduces invasiveness.  Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. The 36/E-Cadherin monoclonal antibody recognizes the cytoplasmic domain of E-Cadherin, regardless of phosphorylation status.  The peptide immunogen was generated from human E-Cadherin aa. 735-883.

Note: Investigators are advised that this antibody has some degree of cross-reactivity to P-Cadherin.

612131 Rev. 2
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
612131 Rev.2
Citations & References
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Development References (7)

  1. Behrens J, Vakaet L, Friis R, et al. Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene.. J Cell Biol. 1993; 120(3):757-66. (Biology). View Reference
  2. Jaksits S, Kriehuber E, Charbonnier AS, Rappersberger K, Stingl G, Maurer D. CD34+ cell-derived CD14+ precursor cells develop into Langerhans cells in a TGF-beta 1-dependent manner. J Immunol. 1999; 163(9):4869-4877. (Clone-specific: Flow cytometry). View Reference
  3. Miyoshi K, Shillingford JM, Smith GH, et al. Signal transducer and activator of transcription (Stat) 5 controls the proliferation and differentiation of mammary alveolar epithelium. J Cell Biol. 2001; 155(4):531-542. (Clone-specific: Immunohistochemistry). View Reference
  4. Sheibani N, Sorenson CM, Frazier WA. Differential modulation of cadherin-mediated cell-cell adhesion by platelet endothelial cell adhesion molecule-1 isoforms through activation of extracellular regulated kinases. Mol Biol Cell. 2000; 11(8):2793-2802. (Clone-specific: Immunofluorescence, Western blot). View Reference
  5. Takeichi M. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.. Development. 1988; 102(4):639-55. (Biology). View Reference
  6. Tamm I, Cardinale I, Kikuchi T, Krueger JG. E-cadherin distribution in interleukin 6-induced cell-cell separation of ductal breast carcinoma cells. Proc Natl Acad Sci U S A. 1994; 91(10):4338-4342. (Biology). View Reference
  7. Weng Z, Xin M, Pablo L, et al. Protection against anoikis and down-regulation of cadherin expression by a regulatable beta-catenin protein. J Biol Chem. 2002; 277(21):18677-18686. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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612131 Rev. 2

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.