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    Purified NA/LE Rat Anti-Mouse CSF-1
    Product Details
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    BD Pharmingen™
    M-CSF
    Mouse (QC Testing)
    Rat IgG1, κ
    Purified mouse CSF-1
    ELISA (Routinely Tested), Neutralization (Tested During Development)
    1.0 mg/ml
    AB_396901
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
    RUO


    Preparation And Storage

    Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    557858 Rev. 2
    Antibody Details
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    5A1

    The 5A1 antibody reacts with the macrophage-specific colony stimulating factor (known as CSF-1 or M-CSF) protein. CSF-1 is a hematopoietic growth factor that regulates the survival, proliferation, and differentiation of mononuclear phagocytes. CSF-1 is found to be produced by a vast number of cells including fibroblasts, stromal cells, osteoblasts,activated T cells, macrophages, and B cells.

    557858 Rev. 2
    Format Details
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    NA/LE
    NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
    NA/LE
    557858 Rev.2
    Citations & References
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    Development References (3)

    1. Lokeshwar BL, Lin HS. A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1). J Immunol Methods. 1989; 123(1):123-129. (Biology). View Reference
    2. Lokeshwar BL, Lin HS. Development and characterization of monoclonal antibodies to murine macrophage colony-stimulating factor. J Immunol. 1988; 15(2):483-488. (Biology). View Reference
    3. Nakoinz I, Lee MT, Weaver JF, Ralph P. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J Immunol. 1990; 145(3):860-864. (Biology). View Reference
    557858 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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