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Purified NA/LE Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein

Purified NA/LE Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein

Clone 9H10.CTLA-4.3 (also known as none)

(RUO)
Purified NA/LE Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein
Inhibition of ConA-induced proliferation of splenic T lymphocytes by Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein. C57BL/6 splenocytes were cultured for three days, either with no stimulation or with 4 µg ConA per 10^6 cells, as indicated. The indicated concentrations of either Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein or a control fusion protein were added to ConA-stimulated cells. Cell proliferation was quantitated by the MTT fluorometric assay. The CTLA-4 fusion protein inhibited ConA-induced cell proliferation in a dose-dependent manner, whereas the control fusion protein had little effect.
Inhibition of ConA-induced proliferation of splenic T lymphocytes by Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein. C57BL/6 splenocytes were cultured for three days, either with no stimulation or with 4 µg ConA per 10^6 cells, as indicated. The indicated concentrations of either Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein or a control fusion protein were added to ConA-stimulated cells. Cell proliferation was quantitated by the MTT fluorometric assay. The CTLA-4 fusion protein inhibited ConA-induced cell proliferation in a dose-dependent manner, whereas the control fusion protein had little effect.
Product Details
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BD Pharmingen™
Mouse IgG2a
Blocking (Routinely Tested)
0.5 mg/ml
AB_10119062
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm filtered. Endotoxin level is ≤0.01 ng/µg of protein.
RUO


Preparation And Storage

This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Avoid multiple freeze-thaws of product.

The fusion protein solution should be stored at -20°C until the vial is opened. Thawed aliquots may be stored for at least 1 week at 4°C.

Recommended Assay Procedures

This fusion protein has been tested by LAL assay for endotoxin level, by SDS-PAGE to assure purity, and by an in vitro T-cell proliferation assay to assure blocking activity.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
552133 Rev. 4
Antibody Details
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9H10.CTLA-4.3

The Non-Cytolytic Mouse CTLA-4 - IgG Fusion Protein is composed of the extracellular domain of mouse CTLA-4 (CD152) fused to a mutant Fc region of mouse IgG2a, which is unable to bind to the C1q complement or to Fc receptors. This chimeric protein binds to CTLA-4's ligands B7-1 (CD80) and B7-2 (CD86) on mouse antigen-presenting cells and blocks their binding to both CTLA-4 and CD28. CTLA-4 (also known as CD152) is a cell-surface Ig-superfamily glycoprotein closely related to the CD28 costimulatory receptor. CTLA-4 is expressed on activated T lymphocytes 2-3 days after stimulation through the T-cell receptor. Whereas CD28 delivers a costimulatory signal required for T-cell activation, CTLA-4 is a negative regulator of cell-mediated immune responses. CTLA-4 IgG Fusion Protein has been shown to prevent allograft rejection and induce donor-specific tolerance, inhibit in vitro responses of splenocytes to ConA; inhibit the spontaneous in vitro and in vivo lymphoproliferation in CTLA-4-deficient mice; and limit superantigen-induced T-cell proliferation, anergy, and secretion of IL-2, IFN-γ, and IL-4, but not TNF-α or IL-10.

552133 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
552133 Rev.4
Citations & References
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Development References (5)

  1. Carreno BM, Collins M. The B7 family of ligands and its receptors: New pathways for costimulation and inhibition of immune responses. Annu Rev Immunol. 2002; 20:29-53. (Biology). View Reference
  2. Gonzalo JA, Delaney T, Corcoran J, Goodearl A, Gutierrez-Ramos JC, Coyle AJ. Cutting edge: the related molecules CD28 and inducible costimulator deliver both unique and complementary signals required for optimal T cell activation. J Immunol. 2001; 166(1):1-5. (Biology). View Reference
  3. Lenschow DJ, Walunas TL, Bluestone JA. CD28/B7 system of T cell costimulation. Annu Rev Immunol. 1996; 14:233-258. (Biology). View Reference
  4. Steurer W, Nickerson PW, Steele AW, Steiger J, Zheng XX, Strom TB. Ex vivo coating of islet cell allografts with murine CTLA4/Fc promotes graft tolerance. J Immunol. 1995; 155(3):1165-1174. (Biology). View Reference
  5. Tivol EA, Boyd SD, McKeon S, et al. CTLA4Ig prevents lymphoproliferation and fatal multiorgan tissue destruction in CTLA-4-deficient mice. J Immunol. 1997; 158(11):5091-5094. (Biology). View Reference
View All (5) View Less
552133 Rev. 4

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.