Preparation And Storage
Recommended Assay Procedures
ELISA Capture: Purified hIL4R-M10.1 anti-human IL-4R antibody (Cat. No. 552472) is useful as a capture antibody for a sandwich ELISA which measures human IL-4R protein levels. Purified hIL4R-M10.1 anti-human IL-4R antibody can be paired with biotinylated hIL4R-M.8.2.2 anti-human IL-4R (Cat. No. 552473) as the detection antibody, with recombinant soluble human IL-4R as the standard. This capture antibody should be titrated between 1 - 4 µg/ml to determine the optimal coating concentration for ELISA. To obtain linear standard curves, doubling dilutions of recombinant human IL-4R ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, see https://www.bdbiosciences.com/en-us/resources/protocols/cytokine-elisa.
Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: IL-1RI, IL-1RII, IL-1α, IL-1ß, IL-2, IL-2sR-α, IL-3, IL-4, IL-5, IL-6, IL-6R gp130, IL-7, IL-8, IL-10, IL-12 p40, IL-12 p70, IL-13, IL-15, TNF-α, TNF-ß, sTNFRI, sTNFRII, GM-CSF, TGFß, IFN γ, CD40L, TRAIL, FasL.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
The soluble human IL-4 receptor, also known as sCD124, is a140-kDa multimeric membrane glycoprotein that binds IL-4 with high affinity. Although many cell types are able to produce soluble IL-4R at low levels, its production is significantly up-regulated in vitro by T cell activation and IL-4. Cytokines secreted by Th2 cells were found to significantly up-regulate soluble IL-4R production following antigenic stimulation. Studies have indicated that the production of sIL-4R in vivo is closely associated with the secretion of IL-4, and are consistent with reports indicating that sIL-4R are involved in the regulation of Il-4 activity during immune response.
Development References (3)
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Chomarat P, Banchereau J. An update on interleukin-4 and its receptor. Eur Cytokine Netw. 1997; 8(4):333-344. (Biology). View Reference
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Fernandez-Botran R, Chilton PM, Ma Y, Windsor JL, Street NE. Control of the production of soluble interleukin-4 receptors: implications in immunoregulation. J Leukoc Biol. 1996; 59(4):499-504. (Biology). View Reference
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Jacobs CA, Lynch DH, Roux ER, et al. Characterization and pharmacokinetic parameters of recombinant soluble interleukin-4 receptor. Blood. 1991; 77(11):2396-2403. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
