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    Purified Mouse Anti-Human IL-9
    Purified Mouse Anti-Human IL-9
    Curve from a sandwich ELISA that measures IL-9 protein levels.  It was generated using the purified MH9A4 antibody as capture antibody, doubling dilutions of recombinant human IL-9 protein as standard and biotinylated MH9A3 antibody as detection antibody.  Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450 - 570 nm.  The standard curve is displayed as the concentration of recombinant human IL-9 in pg/ml versus the microwell absorbance (in circles).
    Purified Mouse Anti-Human IL-9 Purified Mouse Anti-Human IL-9
    Curve from a sandwich ELISA that measures IL-9 protein levels.  It was generated using the purified MH9A4 antibody as capture antibody, doubling dilutions of recombinant human IL-9 protein as standard and biotinylated MH9A3 antibody as detection antibody.  Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450 - 570 nm.  The standard curve is displayed as the concentration of recombinant human IL-9 in pg/ml versus the microwell absorbance (in circles).
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    BD Pharmingen™
    Human (QC Testing)
    Mouse C57BL/6 IgG2b, κ
    Recombinant human IL-9 protein
    ELISA Capture (Routinely Tested)
    0.5 mg/ml
    AB_647279
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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    Recommended Assay Procedures

    This  antibody is useful as a capture antibody in ELISA assays measuring human IL-9 protein levels.  This antibody is non-blocking, it does not block the binding of IL-9 with the IL-9 receptor.  The MH9A4 mAb can be used as a capture antibody in a sandwich ELISA with Biotinylated MH9A3 anti-human IL-9 protein (Cat. No. 558089) as the detector antibody, with recombinant human IL-9 protein as the standard.  The MH9A4 antibody should be titrated between 1-4 µg/ml to determine its optimal ELISA coating concentration.  To obtain linear standard curves, doubling dilutions of the recombinant human IL-9 protein ranging from 1.0 to 125 pg/ml are recommended for inclusion in each ELISA plate.  For specific methodology, see https://www.bdbiosciences.com/en-us/resources/protocols/cytokine-elisa.

    Note: This ELISA antibody pair shows no crossreactivity with the following recombinant human cytokines: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-18, IFNγ, gp130, TNF, 4-1BB. This ELISA antibody pair also shows no cross-reactivity with recombinant mouse IL-9.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
    5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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    558088 Rev. 3
    Antibody Details
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    MH9A4

    The MH9A4 monoclonal antibody reacts with human interleukin-9 (IL-9), 14.1 kDa T cell-derived multifunctional cytokine . IL-9 induces the proliferation and differentiation of various lymphoid (eg, T lymphocytes, B lymphocytes, mast cells, eosinophils, and neutrophils) and hemopoietic cells,  It potentiates the interleukin-4-induced IgG and IgE responses by human B lymphocytes. Further, IL-9 has been implicated in human allergic disorders.

    558088 Rev. 3
    Format Details
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    Purified
    Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
    Purified
    558088 Rev.3
    Citations & References
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    Development References (5)

    1. Demoulin JB, Renauld JC. Interleukin 9 and its receptor: an overview of structure and function. Int Rev Immunol. 1998; 16(3-4):345-364. (Biology). View Reference
    2. Dugas B, Renauld JC, Pène J, et al. Interleukin-9 potentiates the interleukin-4-induced immunoglobulin (IgG, IgM and IgE) production by normal human B lymphocytes. Eur J Immunol. 1993 July; 23(7):1687-1692. (Biology). View Reference
    3. Jenmalm MC, Van Snick J, Cormont F, Salman B. Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children. Clin Exp Allergy. 2001 October; 31(10):1528-1535. (Biology). View Reference
    4. Renauld JC. IL-9. In: Oppenheim JJ, Feldmann M, Durum SK. editors in chief, Joost J. Oppenheim, Marc Feldman ; editors, Scott K. Durum .. et al., ed. Cytokine reference : a compendium of cytokines and other mediators of host defense. London: Academic Press; 2001:155-164.
    5. Renauld JC. New insights into the role of cytokines in asthma. J Clin Pathol. 2001 August; 54(8):577-589. (Biology). View Reference
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    558088 Rev. 3

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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