Preparation And Storage
Recommended Assay Procedures
The biotinylated H32-544 mAb is useful as a detection antibody for a sandwich ELISA that measures human Blys protein when paired with Purified Mouse anti-Human Blys (Cat. No. 558289) as capture antibody and recombinant human Blys protein as standard. The biotinylated H32-544 antibody should be titrated between 0.25 - 2 µg/ml to determine its optimal concentration. To obtain linear standard curves, doubling dilutions of recombinant human Blys protein ranging from 1.56 - 100 ng/ml are recommended. For specific methodology measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors, see protocol "Cytokine ELISA" under "ELISA and ELISPOT" at http://www.bdbiosciences.com/us/s/resources. For this ELISA antibody pair, no cross-reactivity has been observed with the following: human TNF, TNFRI and TNFRII. This ELISA antibody pair shows a limited cross-reactivity with mouse Blys.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Antibody H32-544 reacts with B-lymphocyte stimulator protein (Blys), also known as BAFF (B cell activating factor), is a member of the TNF ligand superfamily. Members of the TNF receptor and ligand superfamily play a critical role in regulating immune responses. Blys is a type II membrane protein expressed on cells of myeloid lineage including monocytes, dendritic cells, and activated T cells. Blys exists in both a membrane-bound and soluble form. In its soluble form, Blys is a potent B cell growth factor in costimulation assays, suggesting a role in monocyte-driven B cell activation. Blys has also been linked to B cell hyperplasia and autoimmunity.
Development References (4)
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Craxton A, Magaletti D, Ryan EJ, and Clark EA. Macrophage- and dendritic cell-dependent regulation of human B-cell proliferation requires the TNF family ligand BAFF. Blood. 2002; 101:4464-4471. (Biology).
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Mackay F, Sarosi I, Xia X-Z, et al. Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice. Proc Natl Acad Sci U S A. 2000; 97:3370-3375. (Biology).
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Mackay F, Shcneider P, Rennert P, and Browing J. BAFF and APRIL: a tutorial on B cell survival. Annu Rev Immunol. 2003; 21:231-264. (Biology: ELISA).
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Moore PA, Belvedere O, Orr A, et al. BlyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science. 1999; 285:260-263. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
