This page has been recently translated and is available in French now.
Ac-DEVD-AFC Caspase-3 Fluorogenic Substrate(RUO)
Activity of recombinant human caspase-3. Ac-DEVD-AFC (MW 729 Daltons) is a synthetic tetrapeptide substrate that is cleaved between D and AFC, releasing the fluorogenic AFC, which is detected by spectrofluorometry. When coupled to an aldehyde group (CHO), the DEVD tetrapeptide functions as a potent inhibitor of caspase activity and can be used to block caspase cleavage of Ac-DEVD-AFC. Left panel: In the presence of active caspase-3, fluorogenic AFC is released from Ac-DEVD-AFC, demonstrating the activity of caspase-3 enzyme. Right panel: In the presence of both active caspase-3 and Ac-DEVD-CHO, fluorogenic AFC is not released, indicating that Ac-DEVD-AFC was not cleaved and that caspase-3 activity was blocked by Ac-DEVD-CHO.
BD Pharmingen™ Ac-DEVD-AFC Caspase-3 Fluorogenic Substrate
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.