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Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor® is a registered trademark of Life Technologies Corporation.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
Companion Products
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The J90-462 monoclonal antibody specifically recognizes CD6. CD6 is a 105-130 kDa type I transmembrane glycoprotein that belongs to the scavenger receptor family. It is expressed on most thymocytes and peripheral T cells. CD6 is highly expressed on CD3-high thymocytes and expressed at lower levels on CD4+ CD8+ double positive and CD4+CD8- or CD4-CD8+ single positive thymocytes. CD6 is also reportedly expressed at low levels by some neurons. CD6 serves as a receptor for CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). CD6 plays a role in adhesion and migration during organogenesis. The CD6 intracellular domain is constitutively phosphorylated which suggests that CD6 may play a signaling role in T cell activation.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.
Development References (6)
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Bowen MA, Bajorath J, D'Egidio M, et al. Characterization of mouse ALCAM (CD166): the CD6-binding domain is conserved in different homologs and mediates cross-species binding.. Eur J Immunol. 1997; 27(6):1469-78. (Biology). View Reference
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Pal A, Romain PL, Singer NG, Fox D, Stavnezer J. Mouse CD6: sequence of cDNA and expression of mRNA.. Immunol Lett. 1996; 49(1-2):133-7. (Biology). View Reference
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Roncagalli R, Hauri S, Fiore F, et al. Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub.. Nat Immunol. 2014; 15(4):384-92. (Biology). View Reference
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Singer NG, Fox DA, Haqqi TM, et al. CD6: expression during development, apoptosis and selection of human and mouse thymocytes.. Int Immunol. 2002; 14(6):585-97. (Biology). View Reference
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Starling GC, Whitney GS, Siadak AW, et al. Characterization of mouse CD6 with novel monoclonal antibodies which enhance the allogeneic mixed leukocyte reaction.. Eur J Immunol. 1996; 26(4):738-46. (Biology). View Reference
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Whitney G, Bowen M, Neubauer M, Aruffo A. Cloning and characterization of murine CD6.. Mol Immunol. 1995; 32(1):89-92. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
