The MHD1-314 monoclonal antibody specifically binds to Delta-Like Protein 1 which is also known as Delta1, DLL1, and DL1. Delta-Like Protein 1 is a type 1 transmembrane glycoprotein which belongs to the Delta/Serrate/Jagged Family of Notch receptor ligands. The Notch family of transmembrane receptors and their ligands play key roles in various developmental and postnatal processes by controlling cell-fate decisions. Delta-Like Protein 1 is expressed by cells within heart, pancreas, brain, muscle and hematopoietic/lymphoid tissues. This Notch ligand functions in the development and postnatal activities of the nervous and skeletal systems. It also functions in the hematopoietic and immune systems, for example, by promoting the differentiation of hematopoietic progenitors into T cell and NK cell precursors. The MHD1-314 antibody reportedly inhibits the development of osteoclasts from cultured peripheral blood monocytes suggesting that Delta-Like Protein 1 positively regulates osteoclastogenesis.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.