The CH3A4A7 monoclonal antibody reacts with an extracellular epitope on human CD344 which is also known as Frizzled family receptor 4 (FZD4) or Frizzled-4. CD344 is a member of the Frizzled family of proteins within the G protein-coupled receptor (GPCR) superfamily. CD344 is a receptor for Wnt proteins and participates in the activation of the Wnt/beta-catenin pathway (canonical Wnt signaling). Patients with familial exudative vitreoretinopathy (FEVR) carry mutations in Frizzled-4. This disease is characterized by retinal degeneration and hearing loss. On tissues, CD344 is almost ubiquitously expressed. CD344 is highly expressed in adult heart and skeletal muscle.
The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.