Skip to main content Skip to navigation
Purified Rat Anti-Mouse CD324 (E-Cadherin)
Purified Rat Anti-Mouse CD324 (E-Cadherin)

Flow cytometric analysis of CD324 (E-Cadherin) expression on mouse testis embryonal carcinoma cells. F9 cells (ATCC CRL-1720) were stained with Purified Rat IgG1, κ Isotype Control (Cat. No. 553922, 555839, or 559072; dashed line histogram) or Purified Rat Anti-Mouse CD324 (E-Cadherin) (Cat. No. 567053; solid line histogram) at 1 μg/test followed by PE Goat Anti-Rat Ig (Cat. No. 550767). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing CD324 (E-Cadherin) expression (or Ig Isotype control staining) was derived from gated events with the light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Flow cytometric analysis of CD324 (E-Cadherin) expression on mouse testis embryonal carcinoma cells. F9 cells (ATCC CRL-1720) were stained with Purified Rat IgG1, κ Isotype Control (Cat. No. 553922, 555839, or 559072; dashed line histogram) or Purified Rat Anti-Mouse CD324 (E-Cadherin) (Cat. No. 567053; solid line histogram) at 1 μg/test followed by PE Goat Anti-Rat Ig (Cat. No. 550767). DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing CD324 (E-Cadherin) expression (or Ig Isotype control staining) was derived from gated events with the light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD FACSCelesta™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
ARC-1; Cdh1; E-cad; Ecad; UVO; cadherin-1; epithelial cadherin; uvomorulin
Mouse (QC Testing), Human (Tested in Development)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Mouse teratocarcinoma Cell Line
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-paraffin (Reported)
0.5 mg/ml
AB_2870020
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
567053 Rev. 1
Antibody Details
Down Arrow Up Arrow
DECMA-1

The DECMA-1 monoclonal antibody specifically recognizes the extracellular domain of mouse E-Cadherin (CD324). E-Cadherin is a 120-kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-to-cell adhesion. These E-Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces their invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Pluripotent stem cells express E-Cadherin. Upon differentiation, an epithelial to mesenchymal transition results in the loss of E-cadherin expression and a gain in the expression of N-cadherin. The DECMA-1 mAb recognizes the membrane proximal part of the extracellular region of E-Cadherin and blocks E-Cadherin-mediated aggregation of cells. It has been reported to cross-react with E-Cadherin in humans, as well as several other species. However, the human cross-reactivity was weak when tested by flow cytometry on the MCF-7 breast cancer cell line in comparison to BD Biosciences' Anti-Human DECMA-1 mAb 67A4.

567053 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
567053 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (9)

  1. Batchuluun K, Azuma M, Yashiro T, Kikuchi M. Notch signaling-mediated cell-to-cell interaction is dependent on E-cadherin adhesion in adult rat anterior pituitary.. Cell Tissue Res. 2017; 368(1):125-133. (Clone-specific: Immunohistochemistry). View Reference
  2. Brouxhon SM, Kyrkanides S, Teng X, et al. Monoclonal antibody against the ectodomain of E-cadherin (DECMA-1) suppresses breast carcinogenesis: involvement of the HER/PI3K/Akt/mTOR and IAP pathways. Clin Cancer Res. 2013; 19(12):3234-46. (Clone-specific: Functional assay). View Reference
  3. Mohri Y. Prognostic significance of E-cadherin expression in human colorectal cancer tissue.. Surg Today. 1997; 27(7):606-12. (Clone-specific: Immunohistochemistry). View Reference
  4. Ozawa M, Hoschützky H, Herrenknecht K, Kemler R. A possible new adhesive site in the cell-adhesion molecule uvomorulin.. Mech Dev. 1990; 33(1):49-56. (Clone-specific: Immunofluorescence). View Reference
  5. Schuh R, Vestweber D, Riede I, et al. Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.. Proc Natl Acad Sci USA. 1986; 83(5):1364-8. (Clone-specific: Immunoaffinity chromatography). View Reference
  6. Sugiyama D, Joshi A, Kulkeaw K, et al. A Transcriptional Switch Point During Hematopoietic Stem and Progenitor Cell Ontogeny.. Stem Cells Dev. 2017; 26(5):314-327. (Biology). View Reference
  7. Takeichi M. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.. Development. 1988; 102(4):639-55. (Biology). View Reference
  8. Vestweber D, Kemler R. Identification of a putative cell adhesion domain of uvomorulin.. EMBO J. 1985; 4(13A):3393-8. (Immunogen: Blocking, Immunofluorescence, Immunoprecipitation). View Reference
  9. Vleminckx K, Vakaet L, Mareel M, Fiers W, van Roy F. Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role.. Cell. 1991; 66(1):107-19. (Biology). View Reference
View All (9) View Less
567053 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.