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Purified Rat Anti-Mouse CD244.1
Purified Rat Anti-Mouse CD244.1

Two-color analysis of CD244.1 expression on splenic NK cells. BALB/c splenocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-Mouse CD244.1 (Cat. No. 552421; right panel), followed by FITC Mouse Anti-Rat IgG2b (Cat. No. 553900) then PE Rat Anti-Mouse CD49b (Cat. No. 553858). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable splenocytes. Flow cytometry was performed on a BD FACSCalibur™.

Two-color analysis of CD244.1 expression on splenic NK cells. BALB/c splenocytes were stained with either Purified Rat IgG2b, κ Isotype Control (Cat. No. 553986; left panel) or Purified Rat Anti-Mouse CD244.1 (Cat. No. 552421; right panel), followed by FITC Mouse Anti-Rat IgG2b (Cat. No. 553900) then PE Rat Anti-Mouse CD49b (Cat. No. 553858). The fluorescence contour plots were derived from gated events based on the forward and side light-scattering of viable splenocytes. Flow cytometry was performed on a BD FACSCalibur™.

Product Details
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BD Pharmingen™
2B4 BALB Alloantigen; Cd244; Ly90; NAIL; Nmrk; SLAMF4
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2b, κ
Mouse MHC-unrestricted killer hybridoma 7D4
Flow cytometry (Routinely Tested), Immunoprecipitation, Induction (Reported)
0.5 mg/ml
AB_394385
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
552421 Rev. 4
Antibody Details
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C9.1

CD244 (also known as 2B4 Antigen) is a member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF). It is expressed on all natural killer (NK) cells, IL-2–activated NK cells (LAK), and a subset of T lymphocytes which mediate non-MHC–restricted cytotoxicity. The C9.1 antibody specifically recognizes CD244.1, the 2B4 alloantigen which is expressed by certain cells in most strains tested (A/J, AKR/N, BALB/c, CBA/N, C3H/He, DBA/1, DBA/2, NZB/N, SJL/J, 129/Svj), but not by cells from C57BL/6 or C58/J mice. The expression of the CD244.1 alloantigen on B lymphocytes that was originally described, has not been confirmed in later studies. The extracellular domain of CD244 is a ligand for another CD2 IgSF member, CD48.

552421 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
552421 Rev.4
Citations & References
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Development References (7)

  1. Brown MH, Boles K, van der Merwe PA, Kumar V, Mathew PA, Barclay AN. 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. J Exp Med. 1998; 188(11):2083-2090. (Biology). View Reference
  2. Davis SJ, van der Merwe PA. The structure and ligand interactions of CD2: implications for T-cell function. Immunol Today. 1996; 17(4):177-187. (Biology). View Reference
  3. Kubota K, Katoh H, Muguruma K, Koyama K. Characterization of a surface membrane molecule expressed by natural killer cells in most inbred mouse strains: monoclonal antibody C9.1 identifies an allelic form of the 2B4 antigen. Immunology. 1999; 96(3):491-497. (Immunogen). View Reference
  4. Kubota K. A killer cell protective antigen expressed by MHC-unrestricted killer hybridomas. Cell Immunol. 1997; 181(1):50-58. (Immunogen). View Reference
  5. Kumaresan PR, Huynh VT, Mathew PA. Polymorphism in the 2B4 gene of inbred mouse strains. Immunogenetics. 2000; 51(8-9):758-761. (Biology). View Reference
  6. Latchman Y, McKay PF, Reiser H. Identification of the 2B4 molecule as a counter-receptor for CD48. J Immunol. 1998; 161(11):5809-5812. (Biology). View Reference
  7. Mathew PA, Garni-Wagner BA, Land K, et al. Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells. J Immunol. 1993; 151(10):5328-5337. (Biology). View Reference
View All (7) View Less
552421 Rev. 4

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.