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    Purified NA/LE Rat Anti-Mouse GM-CSF
    Product Details
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    BD Pharmingen™
    Mouse (QC Testing)
    Rat IgG2a
    Recombinant Mouse GM-CSF
    ELISA (Routinely Tested), Intracellular staining (flow cytometry), Neutralization (Tested During Development)
    1.0 mg/ml
    AB_395369
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
    RUO


    Preparation And Storage

    Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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    Recommended Assay Procedures

    ELISA:  Purified Rat Anti-Mouse GM-CSF antibody (Clone MP1-22E9, Cat. No. 554403 or 554404) can be useful as a capture antibody for sandwich ELISA for measuring mouse GM-CSF protein levels.  Purified Rat Anti-Mouse GM-CSF can be paired with Biotin Rat Anti-Mouse GM-CSF (Cat. No. 554407, clone MP1-31G6) as the detection antibody, with recombinant mouse GM-CSF protein (Cat. No. 554586) as the standard. For testing mouse GM-CSF in complex biological fluids, such as serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse GM-CSF ELISA Set (Cat. No. 555167).

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    554403 Rev. 2
    Antibody Details
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    MP1-22E9

    The MP1-22E9 monoclonal antibody specifically binds to mouse Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF). The immunogen used to generate the MP1-22E9 hybridoma was yeast-expressed recombinant mouse GM-CSF. This is a neutralizing antibody.

    554403 Rev. 2
    Format Details
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    NA/LE
    NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
    NA/LE
    554403 Rev.2
    Citations & References
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    Development References (4)

    1. Nabel G, Fresno M, Chessman A, Cantor H. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell. 1981; 23(1):19-28. (Biology). View Reference
    2. Nozaki S, Abrams JS, Pearce MK, Sauder DN. Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes. J Invest Dermatol. 1991 July; 97(1):10-14. (Biology). View Reference
    3. Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Biology). View Reference
    4. Suda T, O'Garra A, MacNeil I, Fischer M, Bond MW, Zlotnik A. Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas. Cell Immunol. 1990; 129(1):228-240. (Biology). View Reference
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    554403 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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