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Purified NA/LE Mouse Anti-Human HLA-ABC

Purified NA/LE Mouse Anti-Human HLA-ABC

Clone DX17

(RUO)
Purified NA/LE Mouse Anti-Human HLA-ABC
Profile of human HLA-A,B,C, (DX17) reactivity on peripheral blood lymphocytes analyzed by flow cytometry (solid line) compared to isotype control (dashed line). Second step staining with Cat. No. 555988.
Profile of human HLA-A,B,C, (DX17) reactivity on peripheral blood lymphocytes analyzed by flow cytometry (solid line) compared to isotype control (dashed line). Second step staining with Cat. No. 555988.
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Polyclonal Human NK Cell Line
Flow cytometry (Routinely Tested), Blocking (Reported)
1.0 mg/ml
AB_1645552
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560187 Rev. 1
Antibody Details
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DX17

The DX17 monoclonal antibody reacts with a monomorphic epitope expressed on all HLA (human leukocyte antigen) class I molecules examined.  DX17 immunoprecipitates HLA class I heavy chains (45 kDa) and β2-microglobulin (12kDa) from radiolabeled human cell lines.  HLA is determined by a complex segment of the short arm of chromosome 6 and there are many human HLAs encoded in this segment. The antigenic agglomerate is called MHC, for major histocompatibility complex. Examples of class I loci are HLA-A, -B, and -C, which are serologically assayed; class II loci, e.g., HLA-D/DR and DC1, are tested by lymphocytotoxic methods.

560187 Rev. 1
Citations & References
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Development References (4)

  1. Bach FH, Amos DB. Hu-1: Major histocompatibility locus in man. Science. 1967; 156(781):1506-1508. (Biology). View Reference
  2. Gumperz JE, Litwin V, Phillips JH, Lanier LL, Parham P. The Bw4 public epitope of HLA-B molecules confers reactivity with natural killer cell clones that express NKB1, a putative HLA receptor. J Exp Med. 1995; 181(3):1133-1144. (Biology). View Reference
  3. Lanier LL, Gumperz JE, Parham P, Melero I, López-Botet M, Phillips JH. The NKB1 and HP-3E4 NK cells receptors are structurally distinct glycoproteins and independently recognize polymorphic HLA-B and HLA-C molecules. J Immunol. 1995; 154(7):3320-3327. (Clone-specific). View Reference
  4. Morton CC, Kirsch IR, Nance WE, Evans GA, Korman AJ, Strominger JL. Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization. Proc Natl Acad Sci U S A. 1984; 81(9):2816-2820. (Biology). View Reference
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560187 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.