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Purified NA/LE Hamster Anti-Mouse CD51
Product Details
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BD Pharmingen™
Integrin αv chain
Mouse (QC Testing)
Armenian Hamster IgG3, λ3
Mouse C3H/HeN dendritic epidermal T cell line Y93A Cell Line
Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)
1.0 mg/ml
AB_394730
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.1 EU/µg (≤0.01 ng/µg) of protein as determined by the LAL assay.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553241 Rev. 16
Antibody Details
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H9.2B8

The H9.2B8 antibody reacts with the 140-kDa integrin αv chain (CD51). Heterodimers of CD51 with several integrin β chains function as receptors for extracellular matrix (ECM) proteins. CD51/CD61 (αvβ3 integrin, vitronectin receptor) mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31 (PECAM-1).  It is expressed on activated T lymphocytes, polymorphonuclear granulocytes, blastocysts, and osteoclasts. We have been unable to detect CD51 on mouse platelets using either mAb H9.2B8 or RMV-7.  CD51 also forms heterodimers with integrin β1 (CD29) and β5, β6, and α8 chains. αv integrins have diverse functions in development and homeostasis.  H9.2B8 antibody binds to an epitope of CD51 near or at the receptor-ligand binding site(s), as evidenced by its ability to synergistically participate in blocking of the adherence of vitronectin receptor-bearing cells to ECM proteins.  This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.

553241 Rev. 16
Format Details
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NA/LE
NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
NA/LE
553241 Rev.16
Citations & References
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Development References (9)

  1. Bader BL, Rayburn H, Crowley D, Hynes RO. Extensive vasculogenesis, angiogenesis, and organogenesis precede lethality in mice lacking all alpha v integrins. Cell. 1998; 95(4):507-519. (Biology). View Reference
  2. Frieser M, Hallmann R, Johansson S, Vestweber D, Goodman SL, Sorokin L. Mouse polymorphonuclear granulocyte binding to extracellular matrix molecules involves beta 1 integrins. Eur J Immunol. 1996; 26(12):3127-3136. (Biology). View Reference
  3. Maxfield SR, Moulder K, Koning F, et al. Murine T cells express a cell surface receptor for multiple extracellular matrix proteins. Identification and characterization with monoclonal antibodies. J Exp Med. 1989 June; 169(6):2173-2190. (Immunogen: Blocking, Immunoprecipitation). View Reference
  4. Moulder K, Roberts K, Shevach EM, Coligan JE. The mouse vitronectin receptor is a T cell activation antigen. J Exp Med. 1991; 173(2):343-347. (Clone-specific: Blocking, Immunoprecipitation). View Reference
  5. Nakamura I, Pilkington MF, Lakkakorpi PT, et al. Role of alpha(v)beta(3) integrin in osteoclast migration and formation of the sealing zone. J Cell Sci. 1999; 112(22):3985-3993. (Biology). View Reference
  6. Piali L, Hammel P, Uherek C, et al. CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium. J Cell Biol. 1995; 130(2):451-460. (Biology). View Reference
  7. Roberts K, Yokoyama WM, Kehn PJ, Shevach EM. The vitronectin receptor serves as an accessory molecule for the activation of a subset of gamma/delta T cells. J Exp Med. 1991 January; 173(1):231-240. (Biology). View Reference
  8. Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor. J Biol Chem. 1995; 270(19):11522-11531. (Biology). View Reference
  9. Takahashi K, Nakamura T, Koyanagi M, et al. A murine very late activation antigen-like extracellular matrix receptor involved in CD2- and lymphocyte function-associated antigen-1-independent killer-target cell interaction. J Immunol. 1990; 145(12):4371-4379. (Biology). View Reference
View All (9) View Less
553241 Rev. 16

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.