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Purified Mouse Anti-Rat Integrin αE2 (CD103)
Purified Mouse Anti-Rat Integrin αE2 (CD103)

Flow cytometric analysis of enriched dendritic cells from rat spleen. LOU rat splenocytes were enriched for dendritic cells and incubated with Purified Mouse Anti-Rat Integrin αE2 (CD103) (Cat. No. 555010; right panel) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999; both panels) then PE Streptavidin (Cat. No. 554061; both panels). The fluorescence dot plots were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACSCalibur™.

Flow cytometric analysis of enriched dendritic cells from rat spleen. LOU rat splenocytes were enriched for dendritic cells and incubated with Purified Mouse Anti-Rat Integrin αE2 (CD103) (Cat. No. 555010; right panel) followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999; both panels) then PE Streptavidin (Cat. No. 554061; both panels). The fluorescence dot plots were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometry was performed on a BD FACSCalibur™.

Product Details
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BD Pharmingen™
Integrin alpha E; Itgae; Integrin αE; CD103
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Density gradient-enriched PVG rat thoracic-duct dendritic cells
Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen, Immunoprecipitation, Western blot (Reported)
0.5 mg/ml
AB_395643
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555010 Rev. 9
Antibody Details
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OX-62

The OX-62 monoclonal antibody recognizes an antigen expressed on dendritic cells, dendritic epidermal T cells (but not RT1B+ Langerhans cells), and intestinal intraepithelial lymphocytes of normal rats and on CD3+ lymphocytes in the spleen and cervical lymph nodes of athymic nude rats. The antigen can be detected by flow cytometric or immunocytochemical analysis of leucocytes and by immunohistochemical staining of epidermal sheets, whole mounts of neural tissue, and frozen sections of lymphoid and nonlymphoid organs. The OX-62 monoclonal antibody immunoprecipitates rat Integrin αE2, a molecule that functions in leucocyte adhesion and homing. Sequence analysis reveals that this molecule is homologous to mouse and human CD103 (Integrin αIEL or αE chain).

555010 Rev. 9
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555010 Rev.9
Citations & References
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Development References (7)

  1. Brenan M, Puklavec M. The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin. J Exp Med. 1992; 175(6):1457-1465. (Immunogen: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
  2. Brenan M, Rees DJ. Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 1997; 27(11):3070-3079. (Clone-specific: Immunofluorescence, Immunohistochemistry, Western blot). View Reference
  3. Chen-Woan M, Delaney CP, Fournier V, et al. In vitro characterization of rat bone marrow-derived dendritic cells and their precursors. J Leukoc Biol. 1996; 59(2):196-207. (Clone-specific: Immunocytochemistry (cytospins)). View Reference
  4. Gieseler R, Hoffmann PR, Kuhn R, et al. Enrichment and characterization of dendritic cells from rat renal mesangium. Scand J Immunol. 1997; 46(6):587-596. (Clone-specific: Immunohistochemistry). View Reference
  5. McMenamin PG. Distribution and phenotype of dendritic cells and resident tissue macrophages in the dura mater, leptomeninges, and choroid plexus of the rat brain as demonstrated in wholemount preparations. J Comp Neurol. 1999; 405(4):553-562. (Clone-specific: Immunofluorescence, Immunohistochemistry). View Reference
  6. Nelson DJ, McMenamin C, McWilliam AS, Brenan M, Holt PG. Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract. J Exp Med. 1994; 179(1):203-212. (Clone-specific: Immunohistochemistry). View Reference
  7. Penfield JG, Wang Y, Li S, et al. Transplant surgery injury recruits recipient MHC class II-positive leukocytes into the kidney. Kidney Int. 1999; 56(5):1759-1769. (Clone-specific: Immunohistochemistry). View Reference
View All (7) View Less
555010 Rev. 9

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.