Purified Mouse Anti-Rabbit CD4

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BD Pharmingen™ Purified Mouse Anti-Rabbit CD4

Name: Purified Mouse Anti-Rabbit CD4
Brand: BD Pharmingen™
SKU: 553625

Clone KEN-4

(RUO)

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Product Details

Brand: BD Pharmingen™

Reactivity: Rabbit (QC Testing)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: Japanese White rabbit thymocytes

Application: Flow cytometry (Routinely Tested), Blocking, Immunohistochemistry-frozen, Immunoprecipitation (Reported)

Concentration: 0.5 mg/ml

RRID: AB_2075556

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

553625 Rev. 6

Antibody Details

KEN-4

The KEN-4 antibody reacts with an antigen identified as CD4 on the majority of thymocytes and a subset of peripheral T lymphocytes. mAb KEN-4 immunoprecipitates, from thymocyte lysate, a major band of 50 kDa and a 42-kDa minor band under both reducing and non-reducing conditions. It also blocks the proliferative response of mesenteric lymph node cells to allogeneic splenocytes.

553625 Rev. 6

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

553625 Rev.6

Citations & References

Development References (1)

Kotani M, Yamamura Y, Tamatani T, Kitamura F, Miyasaka M. Generation and characterization of monoclonal antibodies against rabbit CD4, CD5 and CD11a antigens. J Immunol Methods. 1993 January; 157(1-2):241-252. (Immunogen: Blocking, Immunohistochemistry, Immunoprecipitation). View Reference

553625 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.