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Purified Mouse Anti-Human Wiskott-Aldrich Syndrome Protein
Purified Mouse Anti-Human Wiskott-Aldrich Syndrome Protein

Western Blot analysis of WASP in human histiocytic lymphoma. Lysate from U937 cells (ATCC CRL-1593.2) was probed with Mouse anti-Human WASP monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3).  WASP is identified as a band of 60 kDa.

Western Blot analysis of WASP in human histiocytic lymphoma. Lysate from U937 cells (ATCC CRL-1593.2) was probed with Mouse anti-Human WASP monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3).  WASP is identified as a band of 60 kDa.

Product Details
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BD Pharmingen™
WASP
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human WASP Recombinant Protein
Western blot (Routinely Tested), Fluorescence microscopy, Immunoprecipitation, Intracellular staining (flow cytometry) (Reported)
60 kDa
0.25 mg/ml
AB_396867
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
557773 Rev. 5
Antibody Details
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5A5

Wiskott-Aldrich syndrome (WAS) is an X-linked recessive immunodeficiency disease caused by mutations in the gene encoding WAS protein (WASP).  The disease is characterized by a spectrum of clinical signs, including thrombocytopenia, eczema, susceptibility to opportunistic and pyogenic infections, and B-cell lymphomas associated with Epstein-Barr virus.  Furthermore, patients' blood cells display morphological abnormalities that can be associated with an impaired cytoskeleton.  WASP is a member of a family of highly conserved proteins that link signaling pathways to the actin cytoskeleton.  These members include WASP, N-WASP (neuronal), and SCAR/WAVE isoforms (Suppressor of cAMP Receptor/WASP family Verprolin-homologous protein) that share two main regions of homology: a proline-rich domain and a carboxyl terminal domain that binds to the Arp2/3 complex.  The Arp2/3 complex initiates actin filament assembly in motile cells and formation of the immunological synapse between activated T lymphocytes and antigen-presenting cells.  WASP is a central regulator of the actin cytoskeleton in hematopoietic cells that is itself regulated by multiple signaling pathways.

The 5A5 antibody recognizes human WASP; it does not cross react with N-WASP.  It has been reported to detect WASP in lysates of hematopoietic cells and cell lines, except for neutrophils, from normal donors, but not from a group of patients having mutations of the WAS gene.

557773 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
557773 Rev.5
Citations & References
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Development References (5)

  1. Burns S, Cory GO, Vainchenker W, Thrasher AJ. Mechanisms of WASp-mediated hematologic and immunologic disease. Blood. 2004; 104(12):3454-3462. (Biology).
  2. Caron E. Regulation of Wiskott-Aldrich syndrome protein and related molecules. Curr Opin Cell Biol. 2002; 14:82-87. (Biology). View Reference
  3. Kawai S, Minegishi M, Ohashi Y, et al. Flow cytometric determination of intracytoplasmic Wiskott-Aldrich syndrome protein in peripheral blood lymphocyte subpopulations. J Immunol Methods. 2002; 260:195-205. (Immunogen: Flow cytometry, Immunoprecipitation, Western blot). View Reference
  4. Orange JS, Ramesh N, Remold-O'Donnell, et al. Wiskott-Aldrich syndrome protein is required for NK cell cytotoxicity and colocalizes with actin to NK cell-activatting immunologic synapses. Proc Natl Acad Sci U S A. 2002; 99(17):11351-11356. (Clone-specific: Immunofluorescence, Western blot).
  5. Zeng R, Cannon JL, Abraham RT, et al. SLP-76 coordinates Nck-dependent Wiskott-Aldrich syndrome protein recruitment with Vav-1/Cdc42-dependent Wiskott-Aldrich syndrome protein activation at the T cell-APC contact site. J Immunol. 2003; 171:1360-1368. (Biology).
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557773 Rev. 5

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.