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Purified Mouse Anti-Human Langerin (CD207)
Purified Mouse Anti-Human Langerin (CD207)
Immunohistochemical analysis of Langerin (CD207) expression by Langerhans cells within human skin. Acetone-fixed, frozen human skin sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; Left Image) or Purified Mouse Anti-Human Langerin (CD207) antibody (Cat. No. 564789; Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x.
Immunohistochemical analysis of Langerin (CD207) expression by Langerhans cells within human skin. Acetone-fixed, frozen human skin sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; Left Image) or Purified Mouse Anti-Human Langerin (CD207) antibody (Cat. No. 564789; Right Image). A three-step staining procedure that employed Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin-Horseradish Peroxidase (HRP) (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x.
Product Details
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BD Pharmingen™
CLC4K; CLEC4K; C-type lectin domain family 4 member K
Human (QC Testing)
Mouse IgG1, λ
Human Langerin Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested), Immunohistochemistry (Tested During Development)
0.5 mg/ml
AB_2738951
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
564789 Rev. 1
Antibody Details
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2G3

The 2G3 monoclonal antibody specifically binds to Langerin which is also known as CD207 or C-type lectin domain family 4 member K (CLEC4K). CD207 is a 48 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family.  CD207 is selectively expressed by Langerhans cells, an immature dendritic cell type that is found in the epidermis and mucosal epithelia, and by some other dendritic cell subsets. CD207 is expressed on the cell surface membrane and by intracellular Birbeck granules whose formation depends on CD207. CD207 binds to certain surface carbohydrates that are expressed by microbes. It appears to play a role in the uptake of these microbes for subsequent processing and microbial antigen presentation to T cells.

564789 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
564789 Rev.1
Citations & References
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Development References (5)

  1. Flamar AL, Zurawski S, Scholz F, et al. Noncovalent assembly of anti-dendritic cell antibodies and antigens for evoking immune responses in vitro and in vivo. J Immunol. 2012; 189(5):2645-2655. (Clone-specific: Functional assay). View Reference
  2. Igyarto BZ, Haley K, Ortner D, et al. Skin-resident murine dendritic cell subsets promote distinct and opposing antigen-specific T helper cell responses. Immunity. 2011; 35(2):260-272. (Immunogen: ELISA, Flow cytometry, Fluorescence microscopy, Functional assay, In vivo exacerbation). View Reference
  3. Mayumi N, Watanabe E, Norose Y, et al. E-cadherin interactions are required for Langerhans cell differentiation. Eur J Immunol. 2013; 43(1):270-280. (Biology). View Reference
  4. Valladeau J, Ravel O, Dezutter-Dambuyant C, et al. Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity. 2000; 12(1):71-81. (Biology). View Reference
  5. de Witte L, Nabatov A, Pion M, et al. Langerin is a natural barrier to HIV-1 transmission by Langerhans cells. Nat Med. 2007; 13(3):367-371. (Biology). View Reference
View All (5) View Less
564789 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.