Purified Mouse Anti-Human CD95

BD Pharmingen™ Purified Mouse Anti-Human CD95

Name: Purified Mouse Anti-Human CD95
Brand: BD Pharmingen™
SKU: 550042

Clone EOS9.1

(RUO)

Jurkat cells treated for 6 hours with CD95 (EOS9.1)

Jurkat cells treated for 6 hours with mouse IgM isotype control

Jurkat cells treated for 6 hours with CD95 (EOS9.1)

Jurkat cells treated for 6 hours with mouse IgM isotype control

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Product Details

Brand: BD Pharmingen™

Alternative Name: Fas/APO-1

Reactivity: Human (QC Testing)

Isotype: Mouse IgM, κ

Application: Flow cytometry (Routinely Tested), Fluorescence microscopy, Functional assay (Tested During Development)

Concentration: 0.5 mg/ml

RRID: AB_393542

Storage Buffer: Aqueous buffered solution containing no preservative.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4°C.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Purified Mouse IgM, κ Isotype Control RUO

Size 0.1 mg Cat No. 555581

FITC Goat Anti-Mouse IgG/IgM RUO

Size 0.5 mg Cat No. 555988

550042 Rev. 4

Antibody Details

EOS9.1

Reacts with a 45 kD type I membrane molecule also known as APO-1, or Fas antigen. CD95 belongs to the tumor necrosis factor receptor (TNF-R) family of proteins and is expressed on various cells, including activated T cells and B cells. The interaction of CD95, Fas antigen, with Fas ligand is one of the many mechanisms that lead to apoptosis or programmed cell death. The interaction of CD95 expressing target cells with Fas ligand on cytotoxic T cells induces an apoptotic signal leading to target cell death. It has been reported that apoptosis is the mechanism used by the immune system in clonal deletion of self-reactive T cells during embryological development.

550042 Rev. 4

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

550042 Rev.4

Citations & References

Development References (4)

Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
Krammer PH, Dhein J, Walczak H. The role of APO-1-mediated apoptosis in the immune system. Immunol Rev. 1994; (142):175-191. (Biology). View Reference
Nagata S, Golstein P. The Fas death factor. Science. 1995; 267(5203):1449-1456. (Biology). View Reference
Rouvier E, Luciani MF, Golstein P. Fas involvement in Ca(2+)-independent T cell-mediated cytotoxicity. J Exp Med. 1993; 177(1):195-200. (Biology). View Reference

View All (4)

550042 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.