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Purified Mouse Anti-Human CD88
Product Details
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BD Pharmingen™
C5a Receptor; C5aR; C5a anaphylatoxin receptor
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.5 mg/ml
AB_393705
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550493 Rev. 6
Antibody Details
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D53-1473

The D53-1473 monoclonal antibody specifically recognizes CD88, a 40 kDa polypeptide expressed on granulocytes (high), monocytes (low) and macrophages. CD88 is a G type protein-coupled receptor with seven transmembrane domains. It binds to C5a, the α chain of the complement component C5. C5a is cleaved from C5 during complement activation. The binding of C5a to C5a receptor (C5aR, CD88) on granulocytes leads to various effects including shedding of selectins, upregulation of adhesion molecules, chemotaxis, granule exocytosis and activation of NADPH oxidase. Clone D53-1473 was developed using a known C5aR N-terminal extracellular hydrophilic region (a.a. 1-31) peptide as immunogen. This antibody is useful in inflammation and  chemotaxis research.

This antibody is routinely tested by flow cytometric analysis.  Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

550493 Rev. 6
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550493 Rev.6
Citations & References
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Development References (3)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Morelli A, Larregina A, Chuluyán I, Kolkowski E, Fainboim L. Expression and modulation of C5a receptor (CD88) on skin dendritic cells. Chemotactic effect of C5a on skin migratory dendritic cells. Immunology. 1996; 89(1):126-134. (Biology). View Reference
  3. Morgan EL, Ember JA, Sanderson SD, et al. Anti-C5a receptor antibodies. Characterization of neutralizing antibodies specific for a peptide, C5aR-(9-29), derived from the predicted amino-terminal sequence of the human C5a receptor. J Immunol. 1993; 151(1):377-388. (Biology). View Reference
550493 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.