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Purified Mouse Anti-GFAP
Purified Mouse Anti-GFAP
Immunohistochemistry analysis of GFAP expression on human brain. Formalin-fixed, paraffin embedded section of human brain was stained with Purified Mouse Anti-GFAP (Cat. No. 556329) and visualized using a DAB chromogen and hematoxylin counterstain. Red arrow indicates an astrocyte (positive).
Purified Mouse Anti-GFAP

Flow cytometric analysis of GFAP expression on human brain cell line U373. U373 cells were stained with either Purified Mouse Anti-GFAP (solid line histogram) or Purified Mouse IgG2b, κ Isotype Control (dashed line histogram; Cat. No. 556654), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.

Immunohistochemistry analysis of GFAP expression on human brain. Formalin-fixed, paraffin embedded section of human brain was stained with Purified Mouse Anti-GFAP (Cat. No. 556329) and visualized using a DAB chromogen and hematoxylin counterstain. Red arrow indicates an astrocyte (positive).

Flow cytometric analysis of GFAP expression on human brain cell line U373. U373 cells were stained with either Purified Mouse Anti-GFAP (solid line histogram) or Purified Mouse IgG2b, κ Isotype Control (dashed line histogram; Cat. No. 556654), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550589). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.

Product Details
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BD Pharmingen™
Human (QC Testing), Mouse, Rat, Pig, Dog, Chicken, Rabbit, Cow, Guinea Pig, Sheep (Tested in Development)
Mouse IgG2b
Bovine spinal cord homogenate
Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required) (Tested During Development), Immunohistochemistry-frozen, Western blot (Reported)
50 kDa
0.5 mg/ml
AB_396367
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Pharmingen offers additional GFAP specific antibodies: clone 1B4 (Cat. No. 556328); 4A11 (Cat. No. 556327); clones 1B4, 4A11, 2E1 combined and available as a "cocktail" (Cat. No. 556330). Applications include immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (5-25 µg/ml); and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556329 Rev. 8
Antibody Details
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2E1

GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. BD PharMingen offers a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen for clone 2E1. This antibody has broad species reactivity. It recognizes GFAP in brain homogenates from human, mouse, rat, cow, sheep, dog, pig, rabbit, guinea pig and chicken. 2E1 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells.

556329 Rev. 8
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
556329 Rev.8
Citations & References
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Development References (3)

  1. McLendon RE, Bigner DD. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 1994; 4(3):221-228. (Clone-specific). View Reference
  2. McLendon RE, Burger PC, Pegram CN, Eng LF, Bigner DD. The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues. J Neuropathol Exp Neurol. 1986; 45(6):692-703. (Clone-specific: Immunohistochemistry, Western blot). View Reference
  3. Pegram CN, Eng LF, Wikstrand CJ, McComb RD, Lee YL, Bigner DD. Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species. Neurochem Pathol. 1985; 3(2):119-138. (Clone-specific: Immunohistochemistry). View Reference
556329 Rev. 8

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.