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Purified Hamster Anti-Mouse CD49e
Product Details
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BD Pharmingen™
ITGA5; Integrin α5 chain; VLA-5α-chain; VLA5a VLA-5a; Fnra
Mouse (QC Testing), Rat (Tested in Development)
Armenian Hamster IgG1, κ
Affinity-purified mouse VLA-5 protein (α5β1, CD49e/CD29)
Flow cytometry (Routinely Tested), Activation, Immunoprecipitation, Inhibition (Reported)
0.5 mg/ml
16402
AB_394802
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

For optimal immunofluorescent staining, we recommend preincubation with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) (Cat. No. 553141/553142) for mouse cells or Purified Mouse Anti-Rat CD32 (Cat. No. 550270/550271) for rat cells and use of PE Mouse Anti-Armenian and Syrian Hamster IgG Cocktail (Cat. No. 554056) as second step. For immunohistochemical staining (IHC) of acetone-fixed frozen mouse tissues, we have found that the Purified Rat Anti-Mouse CD49e (Cat. No. 553319) yields more satisfactory results than the HMα5-1 antibody. mAb 5H10-27 is not recommended for IHC of formalin-fixed paraffin-embedded sections. Other reported applications include immunoprecipitation, in vitro activation and inhibition, and in vivo inhibition of VLA-5 functions.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
  5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
553350 Rev. 15
Antibody Details
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HM alpha 5-1

The HMα5-1 antibody specifically recognizes the α5 subunit of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on mouse thymocytes and a variety of mouse cell lines, including pre-B and non-lymphoid cells, but not on mouse splenic or lymph node cells. It also detects rat CD49e on the RBL2H3 basophilic leukemia cell line,peritoneal mast cells, and endothelium, but not on splenocytes. It has been reported that soluble mAb HMα5-1 partially inhibits in vitro binding of a mouse cell line and of rat mast cells to fibronectin and inhibits the enhanced degranulation of IgE-sensitized rat RBL cells induced on fibronectin-coated plates. It has also been observed that plate-bound HMα5-1 antibody enhances the degranulation of IgEsensitized rat RBL-2H3 cells and that subcutaneous injection of HMα5-1 mAb into rats, along with anti-CD49d and anti-CD61 antibodies, inhibits experimentally induced passive cutaneous anaphylaxis.

553350 Rev. 15
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
553350 Rev.15
Citations & References
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Development References (4)

  1. Narumiya S, Abe Y, Kita Y, et al. Pre-B cells adhere to fibronectin via interactions of integrin alpha 5/alpha V with RGDS as well as of integrin alpha 4 with two distinct V region sequences at its different binding sites. Int Immunol. 1994; 6(1):139-147. (Immunogen). View Reference
  2. Noto K, Kato K, Okumura K, Yagita H. Identification and functional characterization of mouse CD29 with a mAb. Int Immunol. 1995; 7(5):835-842. (Clone-specific: Activation, Inhibition). View Reference
  3. Tanaka T, Ohtsuka Y, Yagita H, Shiratori Y, Omata M, Okumura K. Involvement of alpha 1 and alpha 4 integrins in gut mucosal injury of graft-versus-host disease. Int Immunol. 1995; 7(8):1183-1189. (Biology). View Reference
  4. Yasuda M, Hasunuma Y, Adachi H, et al. Expression and function of fibronectin binding integrins on rat mast cells.. Int Immunol. 1995; 7(2):251-8. (Immunogen: Activation, Immunoprecipitation, Inhibition). View Reference
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553350 Rev. 15

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.