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    PE Rat Anti-Mouse IgM
    Product Details
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    BD Pharmingen™
    Ighm; Igh-M; Immunoglobulin M; Igh6; muH; immunoglobulin heavy constant mu
    Mouse (QC Testing)
    Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
    Pooled Mouse Ig
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    AB_394845
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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    Recommended Assay Procedures

    PE Rat Anti-Mouse IgM may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC Rat Anti-Mouse IgM (Cat. No. 553437).

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    Product Notices

    1. An isotype control should be used at the same concentration as the antibody of interest.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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    553409 Rev. 10
    Antibody Details
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    R6-60.2

    The R6-60.2 antibody monoclonal antibody specifically binds to mouse Immunoglobulin M (IgM) of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes. The R6-60.2 antibody has not been shown to stimulate B-cell proliferation.

    553409 Rev. 10
    Format Details
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    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    553409 Rev.10
    Citations & References
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    Development References (6)

    1. Duy C, Yu JJ, Nahar R, et al. BCL6 is critical for the development of a diverse primary B cell repertoire. J Exp Med. 2010; 207(6):1209-1221. (Biology). View Reference
    2. Gavin AL, Duong B, Skog P, et al. δBAFF, a splice isoform of BAFF, opposes full-length BAFF activity in vivo in transgenic mouse models. J Immunol. 2005; 75(1):319-328. (Biology). View Reference
    3. Hennet T, Chui D, Paulson JC, Marth JD. Immune regulation by the ST6Gal sialyltransferase.. Proc Natl Acad Sci USA. 1998; 95(8):4504-9. (Biology). View Reference
    4. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Biology). View Reference
    5. Shapiro HM. Practical Flow Cytometry, 3rd Edition. New York: Wiley-Liss, Inc; 1995:280-281.
    6. Touma M, Keskin DB, Shiroki F, et al. Impaired B cell development and function in the absence of IκBNS. J Immunol. 2011; 187(8):3942-3952. (Biology). View Reference
    View All (6) View Less
    553409 Rev. 10

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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