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PE Rat Anti-Mouse CD200R3
PE Rat Anti-Mouse CD200R3
Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and stained with APC Rat Anti-Mouse CD49b antibody (Cat. No. 560628) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD200R3 antibody (Cat. No. 567291; Right Plot) at 0.5 μg/test. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 (or Ig Isotype control staining) versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD200R3 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142) and stained with APC Rat Anti-Mouse CD49b antibody (Cat. No. 560628) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Plot) or PE Rat Anti-Mouse CD200R3 antibody (Cat. No. 567291; Right Plot) at 0.5 μg/test. A bivariate pseudocolor density plot showing the correlated expression of CD200R3 (or Ig Isotype control staining) versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System equipped with a Yellow-Green Laser. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
CD200 receptor-like 3; CD200RLb; Cd200r3; mCD200RLb
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
Mouse Basophils
Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
567291 Rev. 1
Antibody Details
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Ba13

The Ba13 monoclonal antibody specifically recognizes Cell surface glycoprotein CD200 receptor 3 (CD200R3) which is also known as CD200 cell surface glycoprotein receptor-like b (CD200RLb), CD200 receptor-like 3, and OX-2 Receptor 3. CD200R3 is a single-pass type I transmembrane glycoprotein that is encoded by Cd200r3 (CD200 receptor 3) which belongs to the immunoglobulin superfamily (IgSF). It is expressed on basophils and mast cells that are involved in protective immune responses against parasitic infections and in the development of allergic reactions. Cell surface CD200R3 presents as a disulfide-linked dimeric CD200R-like receptor. The cytoplasmic tail region of CD200R3 contains a positively charged lysine residue which allows this receptor to associate and signal through adaptor proteins such as DAP10, DAP12, CD3z, or FcRγ. CD200R3 functions as an activating receptor to regulate IgE-independent immune responses.

567291 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
567291 Rev.1
Citations & References
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Development References (3)

  1. Kojima T, Obata K, Mukai K, et al. Mast cells and basophils are selectively activated in vitro and in vivo through CD200R3 in an IgE-independent manner.. J Immunol. 2007; 179(10):7093-100. (Biology). View Reference
  2. Nei Y, Obata-Ninomiya K, Tsutsui H, et al. GATA-1 regulates the generation and function of basophils.. Proc Natl Acad Sci U S A. 2013; 110(46):18620-5. (Clone-specific: Flow cytometry). View Reference
  3. Pellefigues C, Mehta P, Prout MS, et al. The Basoph8 Mice Enable an Unbiased Detection and a Conditional Depletion of Basophils. Front Immunol. 2019; 10:2143. (Clone-specific: Flow cytometry). View Reference
567291 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.