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PE Rabbit Anti-Human CD88
PE Rabbit Anti-Human CD88

Flow cytometric analysis and staining profile of antibody C85-4124 on human peripheral blood cell. Lysed whole blood cells were stained with PE Rabbit Anti-Human CD88 ((Cat. No. 552993; Filled histogram), overlayed with autofluorescence control (Open histogram). To demonstrate specificity of staining, C85-4124-PE staining was blocked by pre- incubation of the cells with an 100 fold excess of Purified Rabbit Anti-Human CD88 antibody (Cat. No. 559159), that resulted in 99% blocking of the label (bold line histogram). The histograms were derived from the gated events based on light scattering characteristics of viable granulocytes.

Flow cytometric analysis and staining profile of antibody C85-4124 on human peripheral blood cell. Lysed whole blood cells were stained with PE Rabbit Anti-Human CD88 ((Cat. No. 552993; Filled histogram), overlayed with autofluorescence control (Open histogram). To demonstrate specificity of staining, C85-4124-PE staining was blocked by pre- incubation of the cells with an 100 fold excess of Purified Rabbit Anti-Human CD88 antibody (Cat. No. 559159), that resulted in 99% blocking of the label (bold line histogram). The histograms were derived from the gated events based on light scattering characteristics of viable granulocytes.

Product Details
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BD Pharmingen™
C5a Receptor
Human (QC Testing)
Rabbit IgG
Human C5aR sequence aa. 9-29
Flow cytometry (Routinely Tested)
20 µl
AB_394538
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Recommended Assay Procedures

Immunofluorescent staining and Flow Cytometry: The C85-4124-PE antibody is useful for flow cytometric analysis of CD88/C5aR expressed on cell surfaces.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
552993 Rev. 3
Antibody Details
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C85-4124

The rabbit monoclonal antibody C85-4124 reacts with human CD88, which is also known as C5a anaphylatoxin Receptor (C5a receptor, C5aR). CD88 (C5aR) is a seven-transmembrane-spanning G-protein coupled receptor. Its ligand, C5a/C5a-desArg, is the 10 kDa cleavage product of serum protein C5, generated upon Complement System activation. Ligand binding activates the receptor, and mediates lysosomal enzyme release, cytokine and superoxide production, and chemotaxis. Ligand-occupied receptors get internalized (downregulated). Main cellular expression of CD88 (C5aR) includes granulocytes, monocytes, dendritic cells, astrocytes, and microglia. The extracellular, N-terminal 9-29 amino acids of the human C5aR sequence was used as an immunogen to generate C85-4124 monoclonal antibody from rabbits.

552993 Rev. 3
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
552993 Rev.3
Citations & References
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Development References (5)

  1. Gerard C, Gerard NP. C5a anaphylatoxin and its seven transmembrane-segment receptor. Annu Rev Immunol. 1994; 12:775-808. (Clone-specific: Flow cytometry).
  2. Gerard NP, Gerard C. The chemotactic receptor for human C5a anaphylatoxin. Nature. 1991; 349(6310):614-617. (Immunogen: Flow cytometry). View Reference
  3. Höpken UE, Lu B, Gerard NP, Gerard C. The C5a chemoattractant receptor mediates mucosal defence to infection. Nature. 1996; 383(6595):86-89. (Biology). View Reference
  4. Morgan EL, Ember JA, Sanderson SD, et al. Anti-C5a receptor antibodies. Characterization of neutralizing antibodies specific for a peptide, C5aR-(9-29), derived from the predicted amino-terminal sequence of the human C5a receptor. J Immunol. 1993; 151(1):377-388. (Immunogen: Flow cytometry). View Reference
  5. O'Barr SA, Caguioa J, Gruol D, et al. Neuronal expression of a functional receptor for the C5a complement activation fragment. J Immunol. 2001; 166(6):4154-4162. (Biology).
View All (5) View Less
552993 Rev. 3

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.