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Immunofluorescent staining of mAb OX-41 on bone-marrow cells. Lewis bone marrow cells were incubated simultaneously with either PE-conjugated mouse IgG2a, κ isotype control mAb G155-178 (Cat. No. 553457, left panel) or PE-conjugated mAb OX-41 (right panel) and FITC-conjugated anti-rat CD11b mAb WT.5 (Cat. No. 554982). For analysis, a gate was drawn to include viable cells only (gate not shown) as determined by propidium iodide staining. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ PE Mouse Anti-Rat CD172
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Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
The OX-41 monoclonal antibody specifically reacts with the rat signal-regulatory protein (SIRP). The rat SIRP (CD172) is a 110-120-kDa type 1 transmembrane protein with three extracellular immunoglobulin superfamily domains and two intracellular Immunoreceptor Tyrosinebased Inhibitory Motifs (ITIM). The SIRP ITIMs are phosphorylated in response to activation by growth hormone, epidermal growth factor, and integrin-mediated cell adhesion. The phosphorylated SIRP associates with tyrosine phosphatases SHP-1 and/or SHP-2. In the rat, SIRP is selectively expressed on myeloid cells (monocytes, macrophages, granulocytes, and dendritic cells) and neurons. The ligand for rat SIRP is CD47, which is similar to the interaction of the human homolog SIRPα1 (CD172α) and human CD47. The SIRP/CD47 interaction is thought to be important in maintaining cellular adhesion and possibly promote T-cell activation.
Development References (4)
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Adams S, van der Laan LJ, Vernon-Wilson E, et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J Immunol. 1998; 161(4):1853-1859. (Biology). View Reference
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Robinson AP, White TM, Mason DW. MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries. Immunology. 1986; 57(2):231-237. (Immunogen). View Reference
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Robinson AP, White TM, Mason DW. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology. 1986; 57(2):239-247. (Immunogen). View Reference
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Vernon-Wilson EF, Kee WJ, Willis AC, Barclay AN, Simmons DL, Brown MH. CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1. Eur J Immunol. 2000; 30(8):2130-2137. (Biology). View Reference
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