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PE Mouse Anti-Rat CD172
PE Mouse Anti-Rat CD172

Immunofluorescent staining of mAb OX-41 on bone-marrow cells. Lewis bone marrow cells were incubated simultaneously with either PE-conjugated mouse IgG2a, κ isotype control mAb G155-178 (Cat. No. 553457, left panel) or PE-conjugated mAb OX-41 (right panel) and FITC-conjugated anti-rat CD11b mAb WT.5 (Cat. No. 554982). For analysis, a gate was drawn to include viable cells only (gate not shown) as determined by propidium iodide staining. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Immunofluorescent staining of mAb OX-41 on bone-marrow cells. Lewis bone marrow cells were incubated simultaneously with either PE-conjugated mouse IgG2a, κ isotype control mAb G155-178 (Cat. No. 553457, left panel) or PE-conjugated mAb OX-41 (right panel) and FITC-conjugated anti-rat CD11b mAb WT.5 (Cat. No. 554982). For analysis, a gate was drawn to include viable cells only (gate not shown) as determined by propidium iodide staining. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

Product Details
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BD Pharmingen™
SIRP; Signal-regulatory protein alpha; Sirpa; Sirp-alpha-1; Mfr; Bit
Rat (QC Testing)
Mouse IgG2a, κ
Resident peritoneal cells from cross-bred rat strains
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394366
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
552298 Rev. 2
Antibody Details
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OX-41

The OX-41 monoclonal antibody specifically reacts with the rat signal-regulatory protein (SIRP). The rat SIRP (CD172) is a 110-120-kDa type 1 transmembrane protein with three extracellular immunoglobulin superfamily domains and two intracellular Immunoreceptor Tyrosinebased Inhibitory Motifs (ITIM). The SIRP ITIMs are phosphorylated in response to activation by growth hormone, epidermal growth factor, and integrin-mediated cell adhesion. The phosphorylated SIRP associates with tyrosine phosphatases SHP-1 and/or SHP-2. In the rat, SIRP is selectively expressed on myeloid cells (monocytes, macrophages, granulocytes, and dendritic cells) and neurons. The ligand for rat SIRP is CD47, which is similar to the interaction of the human homolog SIRPα1 (CD172α) and human CD47. The SIRP/CD47 interaction is thought to be important in maintaining cellular adhesion and possibly promote T-cell activation.

552298 Rev. 2
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
552298 Rev.2
Citations & References
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Development References (4)

  1. Adams S, van der Laan LJ, Vernon-Wilson E, et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J Immunol. 1998; 161(4):1853-1859. (Biology). View Reference
  2. Robinson AP, White TM, Mason DW. MRC OX-43: a monoclonal antibody which reacts with all vascular endothelium in the rat except that of brain capillaries. Immunology. 1986; 57(2):231-237. (Immunogen). View Reference
  3. Robinson AP, White TM, Mason DW. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology. 1986; 57(2):239-247. (Immunogen). View Reference
  4. Vernon-Wilson EF, Kee WJ, Willis AC, Barclay AN, Simmons DL, Brown MH. CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRPalpha 1. Eur J Immunol. 2000; 30(8):2130-2137. (Biology). View Reference
View All (4) View Less
552298 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.