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PE Mouse Anti-Human TLR6 (CD286)
PE Mouse Anti-Human TLR6 (CD286)
Two-parameter flow cytometric analysis of TLR6 (CD286) expression on human peripheral blood leucocyte populations. Human peripheral blood cells were first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; Left Plot) or PE Mouse Anti-Human TLR6 (CD286) antibody (Cat. No. 566339; Right Plot). Two-parameter flow-cytometric contour plots showing the correlated expression of TLR6 (CD286) [or Ig Isotype control staining] versus side light-scatter signals were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Two-parameter flow cytometric analysis of TLR6 (CD286) expression on human peripheral blood leucocyte populations. Human peripheral blood cells were first treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes. The leucocytes were then washed and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 559320; Left Plot) or PE Mouse Anti-Human TLR6 (CD286) antibody (Cat. No. 566339; Right Plot). Two-parameter flow-cytometric contour plots showing the correlated expression of TLR6 (CD286) [or Ig Isotype control staining] versus side light-scatter signals were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
Product Details
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BD Pharmingen™
Toll-like receptor 6; CD286
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human TLR6 Transfected Cell Line
Flow cytometry (Routinely Tested)
5 µL/Test
AB_2739696
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566339 Rev. 1
Antibody Details
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TLR6.127

The TLR6.127 monoclonal antibody specifically recognizes Toll-like receptor 6 (TLR6), which is also known as CD286. CD286 belongs to the mammalian Toll-like receptor (TLR) family of pattern recognition receptors that participate in inducing innate and adaptive immune responses to microbial pathogens. TLR6 is a type I transmembrane glycoprotein comprised of an extracellular domain with a large number of leucinerich repeats (LRRs). Its cytoplasmic region contains a signaling Toll/Interleukin-1 Receptor (TIR) domain. TLR6 is expressed on monocytes, neutrophils, and some dendritic cells. In association with TLR2 (CD282), TLR6 recognizes diacylated lipopeptides and peptidoglycans of bacterial or fungal origin. The ligand-bound TLR2:TLR6 complex transduces responses intracellularly through MyD88 and the NF-κB signaling cascade leading to the production of proinflammatory cytokines by responding cells.

        

566339 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566339 Rev.1
Citations & References
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Development References (3)

  1. Nakamura M, Funami K, Komori A, et al. Increased expression of Toll-like receptor 3 in intrahepatic biliary epithelial cells at sites of ductular reaction in diseased livers.. Hepatol Int. 2008; 2(2):222-30. (Clone-specific: Immunohistochemistry). View Reference
  2. Nakao Y, Funami K, Kikkawa S, et al. Surface-expressed TLR6 participates in the recognition of diacylated lipopeptide and peptidoglycan in human cells. J Immunol. 2005; 174(3):1566-1573. (Immunogen: Blocking, Flow cytometry, Fluorescence microscopy, Functional assay, Immunofluorescence, Immunoprecipitation, Inhibition). View Reference
  3. van Bergenhenegouwen J, Plantinga TS, Joosten LA, et al. TLR2 & Co: a critical analysis of the complex interactions between TLR2 and coreceptors.. J Leukoc Biol. 2013; 94(5):885-902. (Biology). View Reference
566339 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.