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PE Mouse Anti-Human PVR (CD155)
PE Mouse Anti-Human PVR (CD155)

Two-parameter flow cytometric analysis of CD155 (PVR) expression on human peripheral blood leucocytes. Human peripheral blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680) or PE Mouse Anti-Human CD155 (PVR) antibody (Cat. No. 566718). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow-cytometric dot plots showing the correlated expression of CD155 (PVR) (or Ig Isotype control staining) versus side light scatter (SSC) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Two-parameter flow cytometric analysis of CD155 (PVR) expression on human peripheral blood leucocytes. Human peripheral blood was stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680) or PE Mouse Anti-Human CD155 (PVR) antibody (Cat. No. 566718). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-parameter flow-cytometric dot plots showing the correlated expression of CD155 (PVR) (or Ig Isotype control staining) versus side light scatter (SSC) signals were derived from gated events with the forward and side light-scatter characteristics of viable leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.

Product Details
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BD Pharmingen™
poliovirus receptor; HVED; nectin-like protein 5; NECL5; Necl-5; PVR; PVS; TAGE4
Human (QC Testing)
Mouse BALB/c IgG1, κ
SK-N-AS neuroblastoma cell line
Flow cytometry (Routinely Tested)
5 µl
5817
AB_2739771
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566718 Rev. 1
Antibody Details
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SKII.4

The SKII.4 monoclonal antibody specifically binds to the Poliovirus Receptor (PVR) which is also known as CD155, or Nectin-like protein 5 (NECL5). PVR is a ~70 kDa nectin-like type I transmembrane glycoprotein that belongs to the PVR-related (PRR) family within the Ig superfamily. In addition to two cell surface PVR isoforms (alpha and delta), two secreted PVR isoforms (beta and gamma) have been reported that share the same three Ig domains but differ in their C-termini. PVR is expressed on monocytes, macrophages, endothelial cells, epithelia cells, CD34+ thymocytes, and neurons. In addition to serving as a receptor for poliovirus and cytomegalovirus, PVR functions as an adhesion molecule involved in cell-cell and cell-matrix adhesion through interaction with CD96 (TACTILE), Nectin 1-3 (CD111, CD112, CD113), CD226, and vitronectin. PVR promotes natural killer (NK) cell adhesion to and lysis of target cells.

        

566718 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566718 Rev.1
Citations & References
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Development References (4)

  1. Freistadt MS, Eberle KE. CD155 (poliovirus receptor) Workshop Panel Report. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1075-1078.
  2. Freistadt MS, Fleit HB, Wimmer E.. Poliovirus receptor on human blood cells: a possible extraneural site of poliovirus replication. Virology. 1993; 195(2):798-803. (Biology). View Reference
  3. Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M. Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).. J Immunol. 2004; 172(7):3994-8. (Immunogen: Flow cytometry, Immunofluorescence). View Reference
  4. Pende D, Bottino C, Castriconi R, et al. PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis.. Mol Immunol. 2005; 42(4):463-9. (Biology). View Reference
View All (4) View Less
566718 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.